High Content Imaging
Automated high-content imaging for fixed, live-cells and histology sections.
High Content Imaging (HCI) combines automated microscopy with advanced image analysis to capture and analyse large volumes of imaging data quickly and reproducibly. HCI is widely used for drug discovery, phenotypic screening, and live-cell imaging experiments.
Applications
- Drug discovery and compound screening
- Phenotypic screening
- Automated multiwell plate imaging
- Long-term live-cell imaging
- Quantitative fluorescence analysis
- Automated image segmentation and classification
- High-throughput microscopy workflows
Instruments
Cell Discoverer 7 Systems
SLIM hosts two Zeiss Cell Discoverer 7 systems located within the Biodiscovery Institute (University Park Campus) and the Medical School at Queen’s Medical Centre (QMC). These advanced automated imaging systems combine the flexibility of a research microscope with the throughput and automation required for large-scale imaging experiments.
Key Features & Capabilities
- Widefield, confocal, and Airyscan imaging modalities
- Super-resolution imaging capability (~140 nm resolution)
- Automated multiwell plate imaging (up to 1536-well plates)
- Brightfield and up to 7-colour fluorescence imaging (LED based)
- Laser lines: 405, 488, 561, and 640 nm
- Automated sample carrier recognition and autofocus
- Integrated environmental control with CO₂ incubation
- Long-term live-cell imaging support
- Robotic loader for unattended imaging over extended periods
- sCMOS camera for fast and sensitive image acquisition
- Automated image segmentation using Zen Blue Intellesis and Arivis AI tools
- Guided acquisition pathways to find regions of interest without user intervention
- Apeer & Arivis cloud workflows for automated image analysis
- Remote instrument access and control via TeamViewer application.
Available Magnifications:
2.5x, 5x, 10x, 20x, 40x, 50x (W) and 100x (W)