Light and Fluorescence Microscopy
A wide range of fluorescence microscopy systems supporting fixed, live-cell, and high-resolution imaging applications.
SLIM provides access to a diverse range of light and fluorescence microscopy systems for brightfield, widefield fluorescence, confocal, live-cell, and super-resolution imaging applications. The facility supports imaging across a broad range of biological samples at different scale from nm all the way to mm and experimental workflows.
Available Microscope Systems
Leica DM4000B
Upright brightfield microscope for image acquisition
- QImaging Micropublisher 3.3 RTV camera
- Objectives: 2.5x, 10x/0.25NA, 20x/0.4NA, 50x oil/0.9NA and 100x oil/1.25NA
Zeiss Axioplan
Upright microscope supporting brightfield, darkfield, polarised and birefringence imaging
- QImaging Micropublisher 5.0 RTV camera
- Objectives: 1.25x, 2.5x, 5x, 10x, 20x and 40x
Zeiss Exciter
- Widefield fluorescence microscope suitable for fixed and live-cell imaging workflows.
- Inverted widefield fluorescence microscope for fixed and live cell imaging
- DAPI, FITC, TRITC and CY5 imaging Brightfield transmitted light and DIC microscopy
- Photometrics Retiga R1 CCD camera
- Motorised stage for stitching and multi-point imaging
- Micro Manager software support
- Objectives: 10x, 20x, 40x, 63x and 100x
DeltaVision ImView
Inverted high sensitivity, live-cell fluorescence microscope
- Fast, low-light multi-channel imaging
- Brightfield transmitted light and DIC microscopy
- Photometrics Prime BSI CMOS camera
- 95% Quantum Efficiency
- Cell tracking, z-stacks and stitching
- Objectives: 10x AIR, 20x AIR, 20x OIL, 40x OIL, 60x W, 60x OIL and 100x OIL
Zeiss AxioImager M2
- Upright motorised widefield microscope
- Brightfield, phase contrast imaging
- Widefield fluorescence imaging
- Separate colour and monochrome cameras, suitable for H&E samples
- Colibri 5 illumination system Z-stack motorised stage
- Tile imaging and stitching
- Objectives: 5x AIR, 10x AIR, 20x AIR, 40x AIR, 63x AIR and 63x OIL or 100x Oil Ph on demand
Zeiss LSM 880C
Inverted Confocal laser scanning microscope (CLSM)
- Fixed and live fluorescence imaging with temperature control
- 3D optical sectioning and reconstruction
- Laser lines: 405, 458, 488, 514, 561 and 633 nm
- Objectives: 10x AIR, 20x AIR, 40x OIL, 63x OIL and 40x W
Zeiss LSM 880F
Inverted Confocal laser scanning microscope (CLSM) with FCS capability
- Fluorescence Correlation Spectroscopy (FCS)
- 32 channel GaAsP photon counting detector
- Suitable for dim fluorescence probes
- Laser lines: 405, 458, 488, 514, 561 and 633 nm
Supported Applications
- Brightfield and fluorescence imaging
- Confocal microscopy (including FRAP, FRET)
- Live-cell imaging 3D image acquisition and reconstruction
- Multi-channel fluorescence imaging Slide scanning and digital pathology workflows
- Cell tracking and time-lapse imaging
Training & Support
SLIM staff provide training, experimental guidance, and technical support for all microscopy systems. Assistance is available for image acquisition, experimental planning, and optimisation of imaging workflows.