糖心原创

Contact

• workRoom C101 The 糖心原创 Medical School
  Queen's Medical Centre
  Nottingham
  NG7 2UH
  UK
• work0115 82 30458
• joelle.goulding@nottingham.ac.uk

Biography

闯辞毛濒濒别 Goulding (n茅e Alcock) graduated from the 糖心原创 with a BSc (Hons) in Genetics (2001) and went on to complete a PhD within the Institute of Genetics (2005). Subsequent postdoctoral work involved investigations into adult stem cells of the cerebellum. 闯辞毛濒濒别 then joined the Cell Signalling Research Group within the 糖心原创 (2007) changing research fields and prompting a strong focus on Molecular Pharmacology. Through studies of endogenous and transgenic expression systems of the adenosine and adrenergic receptors utilising protein-tags, biosensors and fluorescent ligands, 闯辞毛濒濒别 has built up a wide technological knowledge base. In 2009 闯辞毛濒濒别 visited the laboratory of Prof. Carsten Hoffman, Wurzberg Germany, to learn a FlAsh-based FRET technique to study GPCR activation. In 2015 she was awarded a Diploma in Advanced Pharmacology by the British Pharmacological Society. More recently 闯辞毛濒濒别 has gained an Associate Fellowship of the Higher Education Academy.

In April 2017 闯辞毛濒濒别 took up the position of Research Fellow in Advanced Microscopy within COMPARE at Nottingham University. She will be developing research within fluorescence correlation spectroscopy alongside testing methodologies for the study of membrane receptors with the newly acquired Olympus LV200 and PhaseFocus Livecyte.

Expertise Summary

闯辞毛濒濒别 has a passion for imaging techniques and has implemented this passion by developing and optimising strategies to study receptor function.

Imaging expertise

路 Light, confocal and wide-field microscopy (Zeiss LSM 510, 710, 880, Exciter, DeltaVision Elite)

路 TIRF (total internal reflection fluorescence microscopy)

路 FCS (Fluorescence correlation spectroscopy)

路 High-throughput microscopy (Molecular Devices Ultra and Micro confocal plate readers)

路 Perfusion - as used in

路 Data analysis - MetaXpress, Volocity, GraphPad Prism

Additional laboratory-based expertise

路 Cell Signalling assays/biosensors - including cAMP, Ca²⁺, ERK; techniques including FLEX, FRET and BRET

路 Cell culture - Generation of primary cell lines, transient transfection and generation of stable cell lines

路 Radioligand binding, competition and accumulation assays

Teaching Summary

Main responsibilities:

Workshop Convenor

Maths in the Lab - Essential maths skills in day-to-day lab work (Graduate school course)

Key Contributor

A13GPD - GPCR polymorphisms, disease and personalised medicine (Year 3, BMedSci)

B33PPM - GPCR based personalised medicine (Year 3, MPharm)

Research Summary

闯辞毛濒濒别 is the Microscope Officer for the Nottingham side of COMPARE and is currently developing techniques around Fluorescence Correlation Spectroscopy with particular reference to the study of… read more

Selected Publications

• STODDART, LEIGH A., JOHNSTONE, ELIZABETH K. M., WHEAL, AMANDA J., GOULDING, JOELLE, ROBERS, MATTHEW B., MACHLEIDT, THOMAS, WOOD, KEITH V., HILL, STEPHEN J. and PFLEGER, KEVIN D. G., 2015. NATURE METHODS. 12(7), 661-663
• ALCOCK, J., WARREN, A.Y., GOODSON, Y.J., HILL, S.J., KHAN, R.N. and LYMN, J.S., 2010. Biology of Reproduction. 84(4), 646-653
• ALCOCK, J. and SOTTILE, V., 2009. Cell Research. 19(12), 1324-1333
• ALCOCK, JOELLE, LOWE, JAMES, ENGLAND, TIM, BATH, PHILIP and SOTTILE, VIRGINIE, 2009. Neuroscience Letters. 450(2), 114-6

• View all publications

Current Research

闯辞毛濒濒别 is the Microscope Officer for the Nottingham side of COMPARE and is currently developing techniques around Fluorescence Correlation Spectroscopy with particular reference to the study of endogenously expressed receptors.

Check out https://www.imagingscientist.com/ ( https://www.imagingscientist.com/joelle-goulding.html ) for a feel for the role of the Imaging Scientist in todays scientific research.

Microscopy Resources:-

https://www.ibiology.org/online-biology-courses/microscopy-series/

https://www.olympus-lifescience.com/en/microscope-resource/

https://www.microscopyu.com/

http://olympus.magnet.fsu.edu/index.html

http://micro.magnet.fsu.edu/index.html

• KOO, CHEK ZIU, HARRISON, NEALE, NOY, PETER J, SZYROKA, JUSTYNA, MATTHEWS, ALEXANDRA L, HSIA, HUNG-EN, MULLER, STEPHAN A, TUSHAUS, JOHANNA, GOULDING, JOELLE, WILLIS, KATIE, APICELLA, CLARA, CRAGOE, BETHANY, DAVIS, EDWARD, KELES, MURAT, MALINOVA, ANTONIA, MCFARLANE, THOMAS A, MORRISON, PHILIP R, NGUYEN, HANH T H, SYKES, MICHAEL C, AHMED, HAROON, DI MAIO, ALESSANDRO, SEIPOLD, LISA, SAFTIG, PAUL, CULL, ELEANOR, PLIOTAS, CHRISTOS, RUBINSTEIN, ERIC, POULTER, NATALIE S, BRIDDON, STEPHEN J, HOLLIDAY, NICHOLAS D, LICHTENTHALER, STEFAN F and TOMLINSON, MICHAEL G, 2020. The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex. The Journal of biological chemistry.
• SOAVE, MARK, GOULDING, JOELLE, MARKUS, ROBERT, HILL, STEPHEN J and STODDART, LEIGH A, 2020. Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy. Methods in molecular biology (Clifton, N.J.). 2041, 163-181
• GOULDING J, MAY LT and HILL SJ, 2017. Biochemical pharmacology. (In Press.)
• STODDART, LEIGH A., JOHNSTONE, ELIZABETH K. M., WHEAL, AMANDA J., GOULDING, JOELLE, ROBERS, MATTHEW B., MACHLEIDT, THOMAS, WOOD, KEITH V., HILL, STEPHEN J. and PFLEGER, KEVIN D. G., 2015. NATURE METHODS. 12(7), 661-663
• ALCOCK, J., WARREN, A.Y., GOODSON, Y.J., HILL, S.J., KHAN, R.N. and LYMN, J.S., 2010. Biology of Reproduction. 84(4), 646-653
• ALCOCK, JOELLE, LOWE, JAMES, ENGLAND, TIM, BATH, PHILIP and SOTTILE, VIRGINIE, 2009. Neuroscience Letters. 450(2), 114-6
• ALCOCK, J. and SOTTILE, V., 2009. Cell Research. 19(12), 1324-1333
• ALCOCK J, WARREN AY, GOODSON JY, HILL SJ, KHAN RN, LYMN JS, 2009. Oxytocin-induced contractility in human myometrium is inhibited by inhibitors of the enzyme tissue transglutaminase Reproductive Sciences. 16(3), P758
• ALCOCK J, GOODSON JY, WARREN AY, HILL SJ, KHAN RN, LYMN JS, 2009. Phospholipase C isoform expression in the human myometrium is differentially regulated in pregnancy Reproductive Sciences. 16(3), P757
• THOMSON NL, ALCOCK J, GOODSON JY, WARREN AY, HILL SJ, KHAN RN, LYMN JS, 2009. Expression and cellular localisation of tissue transglutaminase II in human myometrium Reproductive Sciences. 16(3), P802
• ALCOCK,, SCOTTING, and SOTTILE,, 2007. Medical Hypotheses.
• SOAVE M, GOULDING J, MARKUS R, HILL SJ and STODDART LA, Methods in molecular biology (Clifton, N.J.). 2041, 163-181