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Christopher Penfold

Lecturer and Course Director for MSc in Molecular Medical Microbiology, Faculty of Medicine & Health Sciences

Personal Details
Teaching and Learning
Research
Publications

Contact

workRoom WA1328C A Floor, West Block
Queen's Medical Centre
Nottingham
NG7 2UH
UK
work0115 8467957
fax0115 8468002
chris.penfold@nottingham.ac.uk

Expertise Summary

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Teaching Summary

Research Summary

Recent Publications

OLUYOMBO, OLUBUKOLA, PENFOLD, CHRISTOPHER N. and DIGGLE, STEPHEN P., 2019. MBIO. 10(1),
KIM YC, TARR AW and PENFOLD CN, 2014. Biochimica et biophysica acta. (In Press.)
BANO S, VANKEMMELBEKE M, PENFOLD CN and JAMES R, 2014. World journal of microbiology & biotechnology. 30(7), 2091-9
LI, C., ZHANG, Y., VANKEMMELBEKE, M., HECHT, O., ALEANIZY, F.S., MACDONALD, C., MOORE, G.R., JAMES, R. and PENFOLD, C.N., 2012. Journal of Biological Chemistry. 287(23), 19048-19057

View all publications

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I teach on:

MBD301: Introduction to Molecular Microbiology
MBD409: Microbial and Molecular techniques (practical sessions)
MBD403: Applied Molecular Microbiology
MBD402: Research Methods
MBD407: Scientififc review of writing

MSc Clinical Microbiology

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MO4MOL: Research Project

BMedsci

Clinical Laboratory Sciences

Current Research

Bacteriocins are protein antibiotics produced by strains of bacteria that are active against related bacteria. We are particularly interested in a group of bacteriocins called colicins that are produced by Escherichia coli and are active against other enterobacteriaceae. They are ~60 KDa proteins that consist of three functional domains that allow the colicin to bind to cell surface receptors, move through the cell envelope and then kill the bacterial cell by, for example, degrading the DNA. We are interested in how the DNase domain of colicin E9 is delivered into the cytoplasm of sensitive cells to exert its antibacterial effect. Antimicrobial peptides Antimicrobial peptides are low molecular weight polypeptides that have narrow spectrum activities. Lysostaphin, for example, is produced by Stapholococcus simulans against Staph. aureus and the clinically important MRSA. Lysostaphin has two domains; a targeting domain which binds to an unknown cell surface receptor of S. aureus and a cytotoxic domain which cleaves a pentaglycine cross bridge in the host cell wall. We are interested in conducting a structure/function study of both the targeting and endopeptidase domains of lysostaphin to determine whether it is possible to produce hybrid molecules with more broad spectrum activities.

OLUYOMBO, OLUBUKOLA, PENFOLD, CHRISTOPHER N. and DIGGLE, STEPHEN P., 2019. MBIO. 10(1),
KIM YC, TARR AW and PENFOLD CN, 2014. Biochimica et biophysica acta. (In Press.)
BANO S, VANKEMMELBEKE M, PENFOLD CN and JAMES R, 2014. World journal of microbiology & biotechnology. 30(7), 2091-9
LI, C., ZHANG, Y., VANKEMMELBEKE, M., HECHT, O., ALEANIZY, F.S., MACDONALD, C., MOORE, G.R., JAMES, R. and PENFOLD, C.N., 2012. Journal of Biological Chemistry. 287(23), 19048-19057
PENFOLD, C.N., LI, C., ZHANG, Y., VANKEMMELBEKE, M. and JAMES, R., 2012. Biochemical Society Transactions. 40(6), 1469-1474
VANKEMMELBEKE M, JAMES R and PENFOLD CN, 2012. Biochemical Society transactions. 40(6), 1517-21
PENFOLD CN, WALKER D and KLEANTHOUS C, 2012. Biochemical Society transactions. 40(6), 1433-7
VANKEMMELBEKE, M., O'SHEA. P., JAMES, R. and PENFOLD, C.N., 2012. PLoS One. 7(9), e46656
HECHT, O., ZHANG, Y., LI, C., PENFOLD, C.N., JAMES, R. and MOORE, G.R., 2010. FEBS Letters. 584(11), 2249-2252
ZHANG, Y., LI, C., VANKEMMELBEKE, M.N., BARDELANG, P., PAOLI, M., PENFOLD, C.N. and JAMES, R., 2010. Molecular Microbiology. 75(3), 623-636
BARDELANG, P., VANKEMMELBEKE, M., ZHANG, Y., JARVIS, H., ANTONIADOU, E., ROCHETTE, S., THOMAS, N.R., PENFOLD, C.N. and JAMES, R., 2009. Biochemical Journal. 418, 615-624
VANKEMMELBEKE, M., ZHANG, Y., MOORE, G.R., KLEANTHOUS, C., PENFOLD, C.N. and JAMES, R., 2009. Journal of Biological Chemistry. 284(28), 18932-18941
ZHANG, Y., VANKEMMELBEKE, M.N., HOLLAND, L.E., WALKER, D.C., JAMES, R. and PENFOLD, C.N., 2008. Journal of Bacteriology. 190(12), 4342-4350
WARFIELD, RACHEL, BARDELANG, PHILIP, SAUNDERS, HELEN, CHAN, WENG C, PENFOLD, CHRISTOPHER, JAMES, RICHARD and THOMAS, NEIL R, 2006. Organic and Biomolecular Chemistry. 4(19), 3626-38
HANDS, S.L., HOLLAND, L.E., VANKEMMELBEKE, M., FRASER, L., MACDONALD, C.J., MOORE, G.R., JAMES, R. and PENFOLD, C.N., 2005. Journal of Bacteriology. 187(19), 6733-6741
TOZAWA, K., MACDONALD, C.J., PENFOLD, C.N., JAMES, R., KLEANTHOUS, C., CLAYDEN, N.J. and MOORE, G.R., 2005. Biochemistry. 44(34), 11496-11507
VANKEMMELBEKE, M., HEALY, B., MOORE, G.R., KLEANTHOUS, C., PENFOLD, C.N. and JAMES, R., 2005. Journal of Bacteriology. 187(14), 4900-4907
PENFOLD, C.N., HEALY, B., HOUSDEN, N.G., BOETZEL, R., VANKEMMELBEKE, M., MOORE, G.R., KLEANTHOUS, C. and JAMES, R., 2004. Journal of Bacteriology. 186(14), 4520-4527
MACDONALD, C. J., TOZAWA, K., COLLINS, E. S., PENFOLD, C. N., JAMES, R., KLEANTHOUS, C., CLAYDEN, N. J. and MOORE, G. R., 2004. Journal of Biomolecular NMR. VOL 30(NUMBER 1), 81-96
LAW, C.J., PENFOLD, C.N., WALKER, D.C., MOORE, G.R., JAMES, R. and KLEANTHOUS, C., 2003. FEBS Letters. 545(2-3), 127-132
COLLINS, E.S., WHITTAKER, S. B.-M., TOZAWA, K., MACDONALD, C., BOETZEL, R., PENFOLD, C.N., REILLY, A., CLAYDEN, N.J., OSBORNE, M.J., HEMMINGS, A.M., KLEANTHOUS, C., JAMES, R. and MOORE, G.R., 2002. Journal of Molecular Biology. 318(3), 787-804
JAMES, R., PENFOLD, C. N., MOORE, G. R. and KLEANTHOUS, C., 2002. Biochimie. VOL 84(NUMBER 5-6), 381-389
CARR, S., PENFOLD, C. N., BAMFORD, V., JAMES, R. and HEMMINGS, A. M., 2000. Structure. VOL 8(NUMBER 1), 57-66
PENFOLD, C. N., GARINOT-SCHNEIDER, C., HEMMINGS, A. M., MOORE, G. R., KLEANTHOUS, C. and JAMES, R., 2000. Molecular Microbiology. VOL 38(PART 3), 639-649
GARINOT-SCHNEIDER, C., PENFOLD, C. N., MOORE, G. R., KLEANTHOUS, C. and JAMES, R., 1997. Identification of residues in the putative TolA box which are essential for the toxicity of the endonuclease toxin colicin E9 Microbiology. VOL 143(NUMBER 9), 2931-2938
PENFOLD, C. N., BENDER, C. L. and TURNER, J. G., 1996. Gene. VOL 183(NUMBER 1/2), 167-173
PENFOLD, C. N., RINGELING, P. L., DAVY, S. L., MOORE, G. R., MCEWAN, A. G. and SPIRO, S., 1996. FEMS Microbiology Letters. VOL 139(NUMBER 2/3), 143-148

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