糖心原创

Contact

• workRoom C214j Biodiscovery Institute
  University Park
  Nottingham
  NG7 2RD
  UK
• work0115 823 1236
• fax0115 823 1230
• chris.denning@nottingham.ac.uk
• /medicine/people/chris.denning

Biography

Chris Denning is a Professor of Stem Cell Biology. and is Director of the 糖心原创 Biodiscovery Institute. He led the University's Research Priority Area in Regenerative Medicine & Stem Cells, and is now lead on the Interdisciplinary Research Cluster in End to End Therapeutics. His lab's interests are in cardiomyocyte (heart cell) differentiation of human pluripotent stem cells (hPSCs: human embryonic stem cells [hESCs] and human induced pluripotent stem cells [hiPSCs]) for use in drug screening and in production of new in vitro models of genetic-based cardiovascular disease. Broadly, the lab works in 3 main areas:

1) Disease modelling. Enabled via human induced pluripotent stem cells with or without additional manipulation by nuclease-mediated gene targeting technologies (including Cas9/CRISPR). As examples, the lab investigate diseases associated with defects in electrophysiology (e.g. long QT syndrome, CPVT, myotonic dystrophy), structure (e.g. myosin heavy chains, alpha-actin), survival (e.g. DMD) and signalling (e.g. b-adrenoceptors, GRK5). We also use gene targeting for knockout and knockin studies.

2) Platforms for cardiotoxicity evaluation. As an example, Chris leads the multinational CRACK-IT consortium, which aims to improve stem cell-cardiomyocyte models in the predictivity of drug-induced cardiotoxicity. The consortium includes 3 additional academic groups (Christine Mummery, Holland; Thomas Eschenhagen, Germany; Godfrey Smith, Scotland), 2 biotechs (ClydeBiosciences; Pluriomics) and Pharma (GlaxoSmithKline). This is facilitated by high throughput phenotyping platforms to assess changes in electrophysiology, calcium and contractility.

3) Automation and interdisciplinary approaches. Over the last 5 years, Chris has developed a 拢2M automation and phenotyping suite. This is embedded in a world-class stem cell culture facility, which includes 25 class II cell culture cabinets. This enables projects such as screening of an 83,000 compound library to find chemistries that improve cardiomyocyte maturity, as well as development of stem cell quality metrics. High throughput approaches have also enabled screening of polymer microarrays to identify chemistries that can alter stem cell and cardiomyocyte fate.

Appointments in brief:

• PhD in Cancer Gene Therapy, Glasgow, 1997;
• Postdoc: Institute for Stem Cell Research, Edinburgh 1997-98;
• Postdoc: Roslin Institute 1998-2001;
• Fellow (2001), Lecturer (2006), Reader (2008), Professor (2011) in Stem Cell Biology; from 2014 Head of Department of Stem Cell Biology, 糖心原创

Education and appointments

First class hons in Biochemistry with Molecular Biology, University of Leeds 1994; PhD in Cancer Gene Therapy at Beatson Institute for Cancer Research, University of Glasgow, 1997; Postdoctoral Research Fellow - gene targeting in mouse ES cells, Institute for Stem Cell Research, University of Edinburgh, 1997-1998; Postdoctoral Research Fellow - gene targeting / cloning in somatic cells, Roslin Institute 1998-2001; Principal Investigator, 糖心原创, 2001-2003; Medical Research Council Fellow, 糖心原创, 2003-2006; Lecturer, 糖心原创, 2006-2008; Reader, 糖心原创 2008-2011; Professor in Stem Cell Biology, 糖心原创 2011-

Expertise Summary

The lab uses automation and high content approaches to investigate impact of drug- and disease-induced perturbation of function of cardiomyocytes derived from stem cells. We have a world-class facility that comprises 25 class II cell culture cabinets, largely dedicated to stem cell biology. Within my own laboratory, specific technical expertise includes:

• Derivation of human induced pluripotent stem cell (hiPSC) lines from healthy individuals of those harbouring genetic lesions
• Culture and cardiomyocyte differentiation from 30-50 different human pluripotent stem cell (hPSC) lines, including hiPSCs and human embryonic stem cell (hESC) lines.
• Genetic manipulation of stem cells, including targeted gene addition, deletion of polymorphic variation using the CRISPR/Cas9 system. Also, over expression and knockdown with lentiviral approaches
• Robotic automation of hPSC culture and differentiation. As examples, we use our bespoke 拢1M Tecan Robotics facility for: 1) Development of quality metrics for automated hPSC scale up and bioprocess; 2) Screening of 83,000 compound library to improve maturity of hPSC-derived cardiomyocytes
• Automated cardiomyocyte phenotyping. Our platforms include: 1) CellOPTIQ for optical imaging of calcium, voltage and contractility; 2) Manual and automated (Nanion Patchliner) for electrophysiology; 3) Multi electrode arrays for field potential analysis; 4) EnVision system for monitoring e.g. whole well calcium flux; 5) CardioExcyte for impedance measurement; 6) Seahorse bioanalyser for 96-well assessment of metabolic activity; 7) Operetta confocal plate reader for high content image analysis. In addition, we work with colleagues at Imperial College London and in Hamburg to use engineered heart tissue approaches to examine contraction in 3D constructs
• Interdisciplinary approaches are a key area for the lab and a strength in Nottingham. For example, we have screened polymer microarrays to identify new substrate chemistries that enable manipulation of stem cell fate and cardiomyocyte function.
• All standard cell and molecular analysis is available, including Q-PCR, FACS, electron microscopy, mass spec etc, while we tend to outsource RNAseq and proteomics

Teaching Summary

In 2007, the 糖心原创 developed a prestigious MSc / Masters Course in Stem Cell Technology. Chris was Course Director and Module Convenor from 2010-15. His main teaching includes

• Human plurupotent stem cells (hPSCs), which include human embryonic stem cells and human induced pluripotent stem cells;
• Maintenance of pluripotency and characterisation of hPSCs;
• Methods of culture and automated scale up, including robotics, multi-well plates, and T-flasks
• Genetic modification of hPSCs
• Cardiomyocyte differentiation
• Disease modelling and drug screening
• International initiatives on hPSCs
• Regulation of hPSCs, including distribution, patents and ethics
• Practical classes in hPSC biology, culture, differentiation and characterisation

Research Summary

Chris' interests are in cardiomyocyte (heart cell) differentiation of human pluripotent stem cells (hPSCs: human embryonic stem cells [hESCs] and human induced pluripotent stem cells [hiPSCs]) for… read more

Selected Publications

• MATSA, E., DIXON, J.E., MEDWAY, C., GEORGIOU, O., PATEL, M.J., MORGAN, K., KEMP, P.J., STANIFORTH, A., MELLOR, I. and DENNING, C., 2013. European Heart Journal. n/a(n/a), n/a
• RAJAMOHAN D, MATSA E, KALRA S, CRUTCHLEY J, PATEL A, GEORGE V and DENNING C, 2013. BioEssays : news and reviews in molecular, cellular and developmental biology. 35(3), 281-98
• DICK, E., MATSA, E., YOUNG, L.E., DARLING, D. and DENNING, C., 2011. Nature Protocols. 6(6), 701-714
• MATSA, E., RAJAMOHAN, D., DICK, E., YOUNG, L., MELLOR, I., STANIFORTH, A. and DENNING, C., 2011. European Heart Journal. 32(8), 952-962

• View all publications

Current Research

Chris' interests are in cardiomyocyte (heart cell) differentiation of human pluripotent stem cells (hPSCs: human embryonic stem cells [hESCs] and human induced pluripotent stem cells [hiPSCs]) for use in drug screening and in production of new in vitro models of genetic-based cardiovascular disease. Broadly, the lab works in 3 main areas:

1) Disease modelling. Enabled via human induced pluripotent stem cells with or without additional manipulation by nuclease-mediated gene targeting technologies (including Cas9/CRISPR). As examples, the lab investigate diseases associated with defects in electrophysiology (e.g. long QT syndrome, CPVT, myotonic dystrophy), structure (e.g. myosin heavy chains, alpha-actin), survival (e.g. DMD) and signalling (e.g. b-adrenoceptors, GRK5). We also use gene targeting for knockout and knockin studies.

Example publications:

• Lin B, Li Y, Han L, Kaplan AD, Ao Y, Kalra S, Bett GC, Rasmusson RL, Denning C, Yang L. Modeling and studying mechanism of dilated cardiomyopathy using induced pluripotent stem cells derived from Duchenne Muscular Dystrophy (DMD) patients. Dis Model Mech. 8(5):457-66, 2015.
• Matsa E, Dixon JE, Medway C, Georgiou O, Patel MJ, Morgan K, Kemp PJ, Staniforth A, Mellor I, Denning C. (2014). Allele-specific RNA interference rescues the long-QT syndrome phenotype in hiPSC cardiomyocytes. Eur Heart J. 35(16):1078-8. PMID: 23470493
• Dick E, Kalra S, Anderson D, George V, Ritso M, Laval SH, Barresi R, Aartsma-Rus A, Lochm眉ller H, Denning C. (2013). Exon skipping and gene transfer restore dystrophin expression in hiPSC-cardiomyocytes harbouring DMD mutations. Stem Cells Dev. 22(20):2714-24. PMID: 23786351
• Andrews et al., (2011). Screening a large, ethnically diverse population of human embryonic stem cells identifies a chromosome 20 minimal amplicon that confers a growth advantage (The International Stem Cell Initiative). Nature Biotechnology. 29(12):1132-44. PMID: 22119741
• Matsa E, Rajamohan D, Dick E, Young L, Mellor I, Staniforth A, Denning C (2011). Drug evaluation in cardiomyocytes derived from human induced pluripotent stem cells carrying a long QT syndrome type 2 mutation. Eur Heart J. 32(8):952-62. PMID: 21367833

2) Platforms for cardiotoxicity evaluation. As an example, Chris leads the multinational CRACK-IT consortium, which aims to improve stem cell-cardiomyocyte models in the predictivity of drug-induced cardiotoxicity. The consortium includes 3 additional academic groups (Christine Mummery, Holland; Thomas Eschenhagen, Germany; Godfrey Smith, Scotland), 2 biotechs (ClydeBiosciences; Pluriomics) and Pharma (GlaxoSmithKline). This is facilitated by high throughput phenotyping platforms to assess changes in electrophysiology, calcium and contractility.

Example publications:

• Rajamohan D, Kalra S, Hoang M, George V, Staniforth A, Russell H, Yang X, Denning C (2016). Automated electrophysiological and pharmacological evaluation of human pluripotent stem cell-derived cardiomyocytes Stem Cells and Development. 25(6):439-52. PMID:26906236
• Berend J van Meer; Henk de Vries; Karl Firth; Jasper van Weerd; Leon G Tertoolen; Marcel Karperien; Pascal Jonkheijm; Chris Denning; Ad IJzerman; Christine Mummery. Small molecule absorption by PDMS in the context of drug response bioassays. Biochemical and Biophysical Research Communications. In Press
• Denning C, Borgdorff V, Crutchley J, Firth KSA, George V, Kalra S, Kondrashov A, Hoang MD, Mosqueira D, Patel A, Prodanov L, Rajamohan D, Skarnes WC, Smith JGW, Young LE (2015). Cardiomyocytes from human pluripotent stem cells: From laboratory curiosity to industrial biomedical platform. Biochim Biophys Acta. Mol Cell Res 2015 pii: S0167-4889(15)00367-5 PMID: 26524115
• Braam SR, Tertoolen L, Casini S, Matsa E, Lu HR, Teisman A, Passier R, Denning C, Gallacher DJ, Towart R, Mummery CL. (2013) Repolarization reserve determines drug responses in human pluripotent stem cell derived cardiomyocytes. Stem Cell Research. 10(1):48-56. PMID: 2308962

3) Automation and interdisciplinary approaches. Over the last 5 years, Chris has developed a 拢2M automation and phenotyping suite. This is embedded in a world-class stem cell culture facility, which includes 25 class II cell culture cabinets. This enables projects such as screening of an 83,000 compound library to find chemistries that improve cardiomyocyte maturity, as well as development of stem cell quality metrics. High throughput approaches have also enabled screening of polymer microarrays to identify chemistries that can alter stem cell and cardiomyocyte fate.

Example publications:

• Dixon JE, Osman G, Morris GE, Markides H, Rotherham M, Bayoussef Z, El-Haj A, Denning C & Shakesheff KM. Highly Efficient Delivery of Functional Cargoes by the Synergistic Effect of GAG Binding Motifs and Cell-Penetrating Peptides. PNAS 2016;113(3):E291-9.
• Patel AK, Celiz AD, Rajamohan D, Anderson DG, Langer R, Davies MC, Alexander MR, and Denning C. A defined synthetic substrate for serum free culture of human stem cell derived cardiomyocytes with improved sarcomeric integrity identified using combinatorial materials microarrays. Biomaterials. 61:257-65, 2015. PMID: 26005764
• Celiz A, Smith JGW, Patel AK, Hook AL, Rajamohan D, George VT, Patel MJ, Epa VC, Singh T, Langer R, Anderson DG, Allen ND, Hay DC, Winkler DA, Barrett DA, Davies MC, Young LE, Denning C*, Alexander MR*. Discovery of a novel polymer for human pluripotent stem cell expansion and multi-lineage differentiation. Advanced Materials. 27(27):4006-12, 2015. PMID: 26033422
• Celiz AD, Smith JGW, Langer R, Anderson DG, Barrett DA, Davies MC, Young LE, Denning C, Alexander MR. The Search for Materials for the Stem Cell Factories of the Future. Nature Materials 13(6):570-9. PMID: 24845996

• MANNHARDT, INGRA, SALEEM, UMBER, MOSQUEIRA, DIOGO, LOOS, MALTE F., ULMER, BARBEL M., LEMOINE, MARC D., LARSSON, CAMILLA, AMEEN, CAROLINE, DE KORTE, TESSA, VLAMING, MARIA L. H., HARRIS, KATE, CLEMENTS, PETER, DENNING, CHRIS, HANSEN, ARNE and ESCHENHAGEN, THOMAS, 2020. STEM CELL REPORTS. 15(4), 983-998
• BODBIN, SARA E, DENNING, CHRIS and MOSQUEIRA, DIOGO, 2020. Transfection of hPSC-Cardiomyocytes Using Viafect Transfection Reagent. Methods and protocols. 3(3),
• KARGARAN, PARISA K., EVANS, JARED M., BODBIN, SARA E., SMITH, JAMES G. W., NELSON, TIMOTHY J., DENNING, CHRIS and MOSQUEIRA, DIOGO, 2020. JOURNAL OF CLINICAL MEDICINE. 9(8),
• SALEEM, UMBER, VAN MEER, BEREND J, KATILI, PUSPITA A, MOHD YUSOF, NURUL A N, MANNHARDT, INGRA, GARCIA, ANA KROTENBERG, TERTOOLEN, LEON, DE KORTE, TESSA, VLAMING, MARIA L H, MCGLYNN, KAREN, NEBEL, JESSICA, BAHINSKI, ANTHONY, HARRIS, KATE, ROSSMAN, ERIC, XU, XIAOPING, BURTON, FRANCIS L, SMITH, GODFREY L, CLEMENTS, PETER, MUMMERY, CHRISTINE L, ESCHENHAGEN, THOMAS, HANSEN, ARNE and DENNING, CHRIS, 2020. Blinded, Multicenter Evaluation of Drug-induced Changes in Contractility Using Human-induced Pluripotent Stem Cell-derived Cardiomyocytes. Toxicological sciences : an official journal of the Society of Toxicology. 176(1), 103-123
• BHAGWAN, JAMIE R., MOSQUEIRA, DIOGO, CHAIREZ-CANTU, KAROLINA, MANNHARDT, INGRA, BODBIN, SARA E., BAKAR, MINE, SMITH, JAMES G. W. and DENNING, CHRIS, 2020. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY. 145, 43-53
• SALEEM, UMBER, MANNHARDT, INGRA, BRAREN, INGKE, DENNING, CHRIS, ESCHENHAGEN, THOMAS and HANSEN, ARNE, 2020. STEM CELL REPORTS. 14(2), 312-324
• ABAKIR, ABDULKADIR, GILES, TOM C., CRISTINI, AGNESE, FOSTER, JEREMY M., DAI, NAN, STARCZAK, MARTA, RUBIO-ROLDAN, ALEJANDRO, LI, MIAOMIAO, ELEFTHERIOU, MARIA, CRUTCHLEY, JAMES, FLATT, LUKE, YOUNG, LORRAINE, GAFFNEY, DANIEL J., DENNING, CHRIS, DALHUS, BJORN, EMES, RICHARD D., GACKOWSKI, DANIEL, CORREA, IVAN R., JR., GARCIA-PEREZ, JOSE L., KLUNGLAND, ARNE, GROMAK, NATALIA and RUZOV, ALEXEY, 2020. NATURE GENETICS. 52(1), 48-+
• DE KORTE, TESSA, KATILI, PUSPITA A., YUSOF, NURUL A. N. MOHD, VAN MEER, BEREND J., SALEEM, UMBER, BURTON, FRANCIS L., SMITH, GODFREY L., CLEMENTS, PETER, MUMMERY, CHRISTINE L., ESCHENHAGEN, THOMAS, HANSEN, ARNE and DENNING, CHRIS, 2020. ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, VOL 60. 60, 529-551
• HOWE, CARMEL L., WEBB, KEVIN F., ABAYZEED, SIDAHMED A., ANDERSON, DAVID J., DENNING, CHRIS and RUSSELL, NOAH A., 2019. JOURNAL OF PHYSICS D-APPLIED PHYSICS. 52(10),
• VAITHILINGAM, J., SANJUAN-ALBERTE, P., CAMPORA, S., RANCE, G.A., JIANG, L., THORPE, J., BURROUGHS, L., TUCK, C.J., DENNING, C., WILDMAN, R.D., HAGUE, R.J.M., ALEXANDER, M.R. and RAWSON, F.J., 2019. ADVANCED FUNCTIONAL MATERIALS. 29(38), 1902016
• DOWDING, K., THOMPSON, J., PIJUAN-GALITO, S., ASHWORTH, J., KJELLEN, L., WILPSHAAR, T., BOVEE, J., ALEXANDER, M. R., SMITH, J., DENNING, C. and MERRY, C., 2019. Investigating glycosaminoglycan developmental diseases using fully defined 3D cell culture environments and human pluripotent stem cells INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY. 100(4), A16-A16
• DE KORTE, TESSA, VAN MEER, BEREND, GARCIA, ANA KROTENBERG, TERTOOLEN, LEON, CLEMENTS, PETER J., BAHINSKI, ANTHONY, ROSSMAN, ERIC I., XU, XIAOPING, TURNER, SAMANTHA, DENNING, CHRIS, VLAMING, MARIJN, BRAAM, STEFAN and MUMMERY, CHRISTINE, 2019. JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS. 99,
• MOSQUEIRA, DIOGO, LIS-SLIMAK, KATARZYNA and DENNING, CHRIS, 2019. High-Throughput Phenotyping Toolkit for Characterizing Cellular Models of Hypertrophic Cardiomyopathy In Vitro. Methods and protocols. 2(4),
• DIKSIN, MOHAMMED, ROWLINSON, JONATHAN, KONDRASHOV, ALEXANDAR, DENNING, CHRIS, HUGHES, JAMIE, CONSTANTIN-TEODO, TIM, CUI, WEI, GELL, CHRIS, ONION, DAVID, CROXALL, NICOLA, SMITH, STUART and RAHMAN, RUMAN, 2019. NEURO-ONCOLOGY. 21, 7-7
• VAN MEER, BEREND J., KROTENBERG, ANA, SALA, LUCA, DAVIS, RICHARD P., ESCHENHAGEN, THOMAS, DENNING, CHRIS, TERTOOLEN, LEON G. J. and MUMMERY, CHRISTINE L., 2019. NATURE COMMUNICATIONS. 10,
• MOSQUEIRA, DIOGO, SMITH, JAMES G. W., BHAGWAN, JAMIE R. and DENNING, CHRIS, 2019. TRENDS IN MOLECULAR MEDICINE. 25(9), 775-790
• SCOTT, E. S., AWFORD, J., HYDE, R., HASSAN, M., HASHMI, L., HARMER, S., FISCHER, P., KELLAM, B., TINKER, A., SMITH, G. and DENNING, C., 2019. Using bioluminescent hiPSC reporter lines and high content screening to identify novel compounds for cardiomyocyte maturation HUMAN GENE THERAPY. 30(2), A8-A8
• OWEN, T. J., SMITH, J. S., BHAGWAN, J. R., MOSQUEIRA, D., SCOTT, E., MANNHARDT, I., BARRIALES-VILLA, R., MONSERRAT, L., HANSEN, A., ESCHENHAGEN, T., HARDING, S. E., MARSTON, S. B. and DENNING, C., 2019. Isogenic pairs of patient-derived hiPSC-CMs with an apical hypertrophic cardiomyopathy/left ventricular non-compaction-associated ACTC1 E99K mutation unveil differential functional deficits HUMAN GENE THERAPY. 30(2), A7-A8
• MOSQUEIRA, D., MANNHARDT, I., BHAGWAN, J. R., LIS-SLIMAK, K., KATILI, P., SCOTT, E., HASSAN, M., PRONDZYNSKI, M., HARMER, S., TINKER, A., SMITH, J. G. W., CARRIER, L., WILLIAMS, P., GAFFNEY, D., ESCHENHAGEN, T., HANSEN, A. and DENNING, C., 2019. CRISPR/Cas9 genome editing in human pluripotent stem cell-cardiomyocytes provides a platform for modeling hypertrophic cardiomyopathy HUMAN GENE THERAPY. 30(2), A5-A5
• DENNING, C., 2019. British Society for Gene and Cell Therapy Autumn Conference Friday 23 November 2018 Regent's University Conference Centre, London, UK Abstracts HUMAN GENE THERAPY. 30(2), A2-A2
• ZHOU, XIN, BUENO-OROVIO, ALFONSO, SCHILLING, RICHARD J., KIRKBY, CLAIRE, DENNING, CHRIS, RAJAMOHAN, DIVYA, BURRAGE, KEVIN, TINKERS, ANDREW, RODRIGUEZ, BLANCA and HARMER, STEPHEN C., 2019. FRONTIERS IN PHYSIOLOGY. 10,
• BHAGWAN, JAMIE R, COLLINS, EMMA, MOSQUEIRA, DIOGO, BAKAR, MINE, JOHNSON, BENJAMIN B, THOMPSON, ALEXANDER, SMITH, JAMES G W and DENNING, CHRIS, 2019. Variable expression and silencing of CRISPR-Cas9 targeted transgenes identifies the AAVS1 locus as not an entirely safe harbour. F1000Research. 8, 1911
• KONDRASHOV A, DUC HOANG M, SMITH JGW, BHAGWAN JR, DUNCAN G, MOSQUEIRA D, MUNOZ MB, VO NTN and DENNING C, 2018. Stem cells and development. 27(6), 391-404
• MOSQUEIRA D, MANNHARDT I, BHAGWAN JR, LIS-SLIMAK K, KATILI P, SCOTT E, HASSAN M, PRONDZYNSKI M, HARMER SC, TINKER A, SMITH JGW, CARRIER L, WILLIAMS PM, GAFFNEY D, ESCHENHAGEN T, HANSEN A and DENNING C, 2018. European heart journal. (In Press.)
• LEWIS LC, LO PC, FOSTER JM, DAI N, CORR脢A IR, DURCZAK PM, DUNCAN G, RAMSAWHOOK A, AITHAL GP, DENNING C, HANNAN NR and RUZOV A, 2017. Epigenetics. 12(4), 277-286
• SALA L, VAN MEER BJ, TERTOOLEN LT, BAKKERS J, BELLIN M, DAVIS RP, DENNING CN, DIEBEN MA, ESCHENHAGEN T, GIACOMELLI E, GRANDELA C, HANSEN A, HOLMAN E, JONGBLOED MR, KAMEL SM, KOOPMAN CD, LACHAUD Q, MANNHARDT I, MOL MP, MOSQUEIRA D, ORLOVA VV, PASSIER R, RIBEIRO MC, SALEEM U, SMITH G, BURTON FLL and MUMMERY CL, 2017. Circulation research. (In Press.)
• DUNCAN G, FIRTH K, GEORGE V, HOANG MD, STANIFORTH A, SMITH G and DENNING C, 2017. Stem cells and development. 26(23), 1695-1705
• RAJAMOHAN, D., KALRA, S., DUC HOANG, M., GEORGE, V., STANIFORTH, A., RUSSELL, H., YANG, X. and DENNING, C., 2016. Stem Cells and Development. 25(6), 439-452
• DIXON, J.E., OSMAN, G., MORRIS, G.E., MARKIDES, H., ROTHERHAM, M., BAYOUSSEF, Z., EL HAJ, A.J., DENNING, C. and SHAKESHEFF, K.M., 2016. Proceedings of the National Academy of Sciences of the United States of America. 113(3), E291-E299
• VAN MEER BJ, DE VRIES H, FIRTH KS, VAN WEERD J, TERTOOLEN LG, KARPERIEN HB, JONKHEIJM P, DENNING C, IJZERMAN AP and MUMMERY CL, 2016. Biochemical and biophysical research communications. 482(2), 323-328
• KALRA S, MONTANARO F and DENNING C, 2016. Journal of neuromuscular diseases. 3(3), 309-332
• GOLDRING C, ANTOINE DJ, BONNER F, CROZIER J, DENNING C, FONTANA RJ, HANLEY NA, HAY DC, INGELMAN-SUNDBERG M, JUHILA S, KITTERINGHAM N, SILVA-LIMA B, NORRIS A, PRIDGEON C, ROSS JA, YOUNG RS, TAGLE D, TORNESI B, VAN DE WATER B, WEAVER RJ, ZHANG F and PARK BK, 2016. Hepatology (Baltimore, Md.). (In Press.)
• ASHA K PATEL, ADAM D CELIZ, DIVYA RAJAMOHAN, ROBERT LANGER, DANIEL G ANDERSON, MARTYN DAVIES, MORGAN R ALEXANDER and CHRIS DENNING, 2015. Biomaterials. 61, 257-265
• CELIZ, A. D., SMITH, J. G. W., PATEL, A. K., HOOK, A. L., RAJAMOHAN, D., GEORGE, V. T., FLATT, L., PATEL, M. J., EPA, V. C., SINGH, T., LANGER, R., ANDERSON, D. G., ALLEN, N. D., HAY, D. C., WINKLER, D. A., BARRETT, D. A., DAVIES, M. C., YOUNG, L. E., DENNING, C. and ALEXANDER, M. R., 2015. Advanced Materials. 27(27), 4006-4012
• DIXON JAMES E, OSMAN GIZEM, MORRIS GAVIN E, MARKIDES HAREKLEA, ROTHERHAM MICHAEL, BAYOUSSEF ZAHIA, EL HAJ ALICIA J., DENNING CHRIS and SHAKESHEFF KEVIN M, 2015. PNAS. 113, E291-E299
• SMITH, J.G.W., CELIZ, A.D., PATEL, A.K., SHORT, R.D., ALEXANDER, M.R. and DENNING, C., 2015. Regenerative Medicine. 10(8), 925-930
• DENNING, C., BORGDORFF, V., CRUTCHLEY, J., FIRTH, K.S.A., GEORGE, V., KALRA, S., KONDRASHOV, A., HOANG, M.D., MOSQUEIRA, D., PATEL, A., PRODANOV, L., RAJAMOHAN, D., SKARNES, W.C., SMITH, J.G.W. and YOUNG, L.E., 2015. Biochimica et Biophysica Acta - Molecular Cell Research.
• PATEL, A.K., TIBBITT, M.W., CELIZ, A.D., DAVIES, M.C., LANGER, R., DENNING, C., ALEXANDER, M.R. and ANDERSON, D.G., 2015. Current Opinion in Solid State and Materials Science.
• LIN, B., LI, Y., HAN, L., KAPLAN, A.D., AO, Y., KALRA, S., BETT, G.C.L., RASMUSSON, R.L., DENNING, C. and YANG, L., 2015. DMM Disease Models and Mechanisms. 8(5), 457-466
• PATEL, A.K., CELIZ, A.D., RAJAMOHAN, D., ANDERSON, D.G., LANGER, R., DAVIES, M.C., ALEXANDER, M.R. and DENNING, C., 2015. Biomaterials. 61, 257-265
• CELIZ AD, SMITH JGW, LANGER R, ANDERSON DG, WINKLER DA, BARRETT DA, DAVIES MC, YOUNG LE, DENNING C and ALEXANDER MR, 2014. Nature Materials. 13(6), 570-9
• CELIZ, A. D., SMITH, J. G. W., PATEL, A. K., LANGER, R., ANDERSON, D. G., BARRETT, D. A., YOUNG, L. E., DAVIES, M. C., DENNING, C. and ALEXANDER, M. R., 2014. BIOMATERIALS SCIENCE. 2(11), 1604-1611
• DIXON, J.E., SHAH, D.A., ROGERS, C., HALL, S., WESTON, N., PARMENTER, C.D.J., MCNALLY, D., DENNING, C. and SHAKESHEFF, K.M., 2014. Proceedings of the National Academy of Sciences of the United States of America. 111(15), 5580-5585
• CELIZ, A.D., SMITH, J.G.W., PATEL, A.K., LANGER, R., ANDERSON, D.G., BARRETT, D.A., YOUNG, L.E., DAVIES, M.C., DENNING, C. and ALEXANDER, M.R., 2014. Biomaterials Science. 2(11), 1604-1611
• CELIZ, A.D., SMITH, J.G.W., LANGER, R., ANDERSON, D.G., WINKLER, D.A., BARRETT, D.A., DAVIES, M.C., YOUNG, L.E., DENNING, C. and ALEXANDER, M.R., 2014. Nature Materials. 13(6), 570-579
• F脰LDES, G., MATSA, E., KRISTON-VIZI, J., LEJA, T., AMISTEN, S., KOLKER, L., KODAGODA, T., DOLATSHAD, N.F., MIOULANE, M., VAUCHEZ, K., AR脕NYI, T., KETTELER, R., SCHNEIDER, M.D., DENNING, C. and HARDING, S.E., 2014. Stem Cell Reports. 3(5), 905-914
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• PASCUT, F.C., KALRA, S., GEORGE, V., WELCH, N., DENNING, C. and NOTINGHER, I., 2013. Biochimica et Biophysica Acta: General Subjects. 1830(6), 3517鈥�3524
• RAJAMOHAN D, MATSA E, KALRA S, CRUTCHLEY J, PATEL A, GEORGE V and DENNING C, 2013. BioEssays : news and reviews in molecular, cellular and developmental biology. 35(3), 281-98
• BRAAM SR, TERTOOLEN L, CASINI S, MATSA E, LU HR, TEISMAN A, PASSIER R, DENNING C, GALLACHER DJ, TOWART R and MUMMERY CL, 2013. Stem cell research. 10(1), 48-56
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• ALMEIDA RD, LOOSE M, SOTTILE V, MATSA E, DENNING C, YOUNG L, JOHNSON AD, GERING M and RUZOV A, 2012. 5-Hydroxymethyl-Cytosine Enrichment Of Non-Committed Cells Is Not A Universal Feature Of Vertebrate Development. Epigenetics : Official Journal Of The Dna Methylation Society. 7(4), 383-389
• PAIK I, SCURR DJ, MORRIS B, HALL G, DENNING C, ALEXANDER MR, SHAKESHEFF KM and DIXON JE, 2012. Biotechnology and bioengineering. 109(10), 2630-41
• MATSA E and DENNING C, 2012. Journal of cardiovascular translational research. 5(5), 581-92
• MATSA, E., RAJAMOHAN, D., DICK, E., YOUNG, L., MELLOR, I., STANIFORTH, A. and DENNING, C., 2011. European Heart Journal. 32(8), 952-962
• DICK, E., MATSA, E., BISPHAM, J., REZA, M., GUGLIERI, M., STANIFORTH, A., WATSON, S., KUMARI, R., LOCHM脺LLER, H., YOUNG, L., DARLING, D. and DENNING, C., 2011. Stem Cell Research. 6(2), 158-167
• PASCUT, F.C., GOH, H.T., WELCH, N., BUTTERY, L.D., DENNING, C. and NOTINGHER, I., 2011. Biophysical Journal. 100(1), 251-259
• PASCUT, F.C., GOH, H.T., GEORGE, V., DENNING, C. and NOTINGHER, I., 2011. Journal of Biomedical Optics. 16(4), 045002
• DICK, E., MATSA, E., YOUNG, L.E., DARLING, D. and DENNING, C., 2011. Nature Protocols. 6(6), 701-714
• INTERNATIONAL STEM CELL INITIATIVE, 2011. Nature Biotechnology. 29(12), 1132-1146
• DIXON, J.E., DICK, E., RAJAMOHAN, D., SHAKESHEFF, K.M. and DENNING, C., 2011. Molecular Therapy. 19(9), 1695-1703
• MAHLSTEDT, M.M., ANDERSON, D., SHARP, J.S., MCGILVRAY, R., MU脩OZ, M.D., BUTTERY, L.D., ALEXANDER, M.R., ROSE, F.R.A.J. and DENNING, C., 2010. Biotechnology and Bioengineering. 105(1), 130-140
• PRIDDLE, HELEN, ALLEGRUCCI, CINZIA, BURRIDGE, PAUL, MUNOZ, MARIA, SMITH, NIGEL M, DEVLIN, LYNDSEY, SJOBLOM, CECILIA, CHAMBERLAIN, SARAH, WATSON, SUE, YOUNG, LORRAINE E and DENNING, CHRIS, 2010. Derivation and characterisation of the human embryonic stem cell lines, NOTT1 and NOTT2. In vitro cellular & developmental biology. Animal. 46, 367-375
• BRAAM SR, DENNING C, MUMMERY CL, 2010. Genetic manipulation of human embryonic stem cells in serum and feeder-free media Methods Mol Biol. 584, 413-23.
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• PRIDDLE, H., GRABOWSKA, A., MORRIS, T., CLARKE, P.A., MCKENZIE, A.J., SOTTILE, V., DENNING, C., YOUNG, L. and WATSON, S., 2009. Cloning and Stem Cells. 11(2), 259-267
• THOMAS, R.J., ANDERSON, D., CHANDRA, A., SMITH, N.M., YOUNG, L.E., WILLIAMS, D. and DENNING, C., 2009. Biotechnology and Bioengineering. 102(6), 1636-1644
• NICOLA KANE, SCOTT MCRAE, CHRIS DENNING, ANDREW H. BAKER, 2009. Viral and non-viral gene delivery and its role in stem cell engineering Drug Discovery Today: Technologies. 5(4), e107-e115
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• BRAAM, S.R., DENNING, C., VAN DEN BRINK, S., KATS, P., HOCHSTENBACH, R., PASSIER, R. and MUMMERY, C.L., 2008. Nature Methods. 5(5), 389-392
• DENNING C* & ANDERSON D., 2008. Cardiomyocytes from human embryonic stem cells as predictors of cardiotoxicity. Drug Discovery Today: Therapeutic Strategies. 5, 223-232
• ANDERSON D, SELF T, MELLOR I, GOH G, HILL S, DENNING C*, 2007. Molecular Therapy. 15, 2027-36
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• PAUL W. BURRIDGE, DAVID ANDERSON, HELEN PRIDDLE, MARIA D. BARBADILLO MU脩OZ, SARAH CHAMBERLAIN, CINZIA ALLEGRUCCI, LORRAINE E. YOUNG AND CHRIS DENNING*, 2007. Stem Cells. 25, 929-38
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• GOH, G., SELF, T., BARBADILLO MU脩OZ, M.D., HALL, I.P., YOUNG, L. and DENNING, C., 2005. Thrombosis and Haemostasis. 94(4), 728-737
• STEELE, W, ALLEGRUCCI, C, SINGH, R, LUCAS, E, PRIDDLE, H, DENNING, C, SINCLAIR, K and YOUNG, L, 2005. Reproductive Biomedicine online. 10(6), 755-66
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