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Kevin Webb

Associate Professor in Applied Optics and Electrophysiology, Faculty of Engineering

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Biography

Dr Kevin Webb is an Associate Professor in Applied Optics and Electrophysiology, in the Optics & Photonics Group of the School of Electrical & Electronic Engineering. Kevin trained at the University of Auckland, New Zealand as an electrophysiologist, patch clamping differentiated fibre cells from the ocular lens - a unique tissue which is itself an optical component. Following a post-doc at University College London working on cell differentiation in the retina, he joined the Institute of Biophysics, Imaging & Optical Science at The 糖心原创in February 2008, applying surface plasmon imaging and electrophysiology to cultures of primary hippocampal neurons. Kevin was awarded a Royal Academy of Engineering/EPSRC Fellowship in 2010 to apply novel imaging and electrophysiological methods to the retina. His current work is using the retinal pigment epithelium as a model system to examine epithelial fluid transport in transparent ocular tissues using a combination of scanning probe microscopy, electrophysiology, and Raman microspectroscopy.

Dr Webb is a member of the Optics and Photonics research group, and directs the Hydrophotonics Research Laboratory.

Expertise Summary

Teaching Summary

Undergraduate courses:

Convenor: EEEE3001 - Third year Project (3rd year, 30 credits, BEng) EEEE4008 - Industrial/Research Orientated Project (4th year, 40 credits, MEng)

Module contributions:

EEEE2046 - Contemporary Engineering Themes

LIFE3001: Cellular and Molecular Neuroscience

Interdisciplinary MSc and final year Projects supervised in EEE, Neuroscience, Biomedical Science

Research Summary

Kevin has an abiding interest in the physiology of transparent tissues of the eye: in particular the cornea, retina, and ocular lens. He was the first to isolate and functionally characterise in… read more

Selected Publications

  • WEBB, K.F and DONALDSON, P.J., 2008. American Journal of Physiology. Cell Physiology. 294(5), C1133-C1145
  • BECKER, D.L, WEBB, K.F, THRASIVOULOU, C, LIN, C-C, NADERSHAHI, R, TSAKIRI, N and COOK, J.E., 2007. Journal of Physiology. 585(Pt 3), 711-9
  • HU, R.G, SUZUKI-KERR, H, WEBB, K.F, RHODES, J.D, COLLISON, D.J, DUNCAN, G and DONALDSON, P.J., 2008. Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens. Experimental eye research. 87(2), 137-46
  • WEBB, K.F. and DONALDSON, P.J., 2009. Investigative Ophthalmology & Visual Science. 50(8), 3808-3818

Current Research

Kevin has an abiding interest in the physiology of transparent tissues of the eye: in particular the cornea, retina, and ocular lens. He was the first to isolate and functionally characterise in vitro the terminally differentiated fibre cells which form the bulk of the ocular lens. His work confirmed at the cellular level the electrophysiological and ion transporting properties of these specialised cells and their role within the connected syncytium which is the ocular lens. Kevin applies novel high resolution imaging and electrophysiological methods to the study of epithelial transport, using model systems which include the cornea, retinal pigment epithelium, lung, and gut. Among the techniques used are confocal laser scanning microscopy, immunofluorescence, optogenetics, scanning ion conductance microscopy, Raman microspectroscopy, and epithelial voltage clamp. Kevin's current work is funded by the BBSRC, entitled "Direct imaging of epithelial fluid transport at the subcellular scale", the Leverhulme Trust ("Watching Plants Drink"), and the US National Science Foundation ("Commercialisation of nanoporous SERS biosensors")

  • LAGANA, S., AKIFJEVS, R., LA ROCCA, A.L., CAIRNS, A., FAY, M.W. and WEBB, K.F., 2023. Investigation of exhaust particles on different TEM grids: A comparison between Graphene oxide and Silicon Nitride grids: SAE International Journal of Fuels and Lubricants SAE International Journal of Fuels and Lubricants. 16(3), XXX-YYY
  • PEREZ-COTA, F., FUENTES-DOMINGUEZ, R., LA CAVERA, S., HARDIMAN, W., YAO, M., SMITH, R. J. and CLARK, M., 2022.
  • MCCLOSKEY, M. C., KASAP, P., AHMAD, S. D., SU, S. H., CHEN, K., MANSOURI, M., RAMESH, N., NISHIHARA, H., BELYAEV, Y., ABHYANKAR, V. V., BEGOLO, S., SINGER, B. H., WEBB, K. F., KURABAYASHI, K., FLAX, J., WAUGH, R. E., ENGELHARDT, B. and MCGRATH, J. L., 2022. Adv Healthc Mater. 11(18), e2200804
  • PEREZ-COTA, FERNANDO, FUENTES-DOMINGUEZ, RAFAEL, HARDIMAN, WILLIAM, YAO, MENGTING, SMITH, RICHARD J and CLARK, MATT, 2022. Picosecond ultrasonics for cell imaging and characterisation
  • PASCUT, F. C., COUVREUR, V., DIETRICH, D., LEFTLEY, N., REYT, G., BOURSIAC, Y., CALVO-POLANCO, M., CASIMIRO, I., MAUREL, C., SALT, D. E., DRAYE, X., WELLS, D. M., BENNETT, M. J. and WEBB, K. F., 2021. Nat Commun. 12(1), 4682
  • HARUN, M. S. R., MARSH, V., ELSAIED, N. A., WEBB, K. F. and ELSHEIKHA, H. M., 2020. Brain Res. 1746, 147002
  • HOWE, C. L., WEBB, K. F., ABAYZEED, S. A., ANDERSON, D. J., DENNING, C. and RUSSELL, N. A., 2019. Journal of Physics D: Applied Physics. 52(10),
  • PETROVA R.S., WEBB K.F., VAGHEFI E., WALKER K., SCHEY K.L. and DONALDSON P.J., 2018. American Journal of Physiology: Cell Physiology. 314(2), C191-C201
  • HENRY, S., WILLIAMS, E., BARR, K., KORCHAGINA, E., TUZIKOV, A., ILYUSHINA, N., ABAYZEED, S. A., WEBB, K. F. and BOVIN, N., 2018. Scientific Reports. 8(1),
  • MADEJSKI, G., LUCAS, K., PASCUT, F. C., WEBB, K. F. and MCGRATH, J. L., 2018. Membranes. 8(2),
  • MADEJSKI, G., LUCAS, K., PASCUT, F. C., WEBB, K. F. and MCGRATH, J. L., 2018. Membranes (Basel). 8(2),
  • SMITH, RICHARD J, MORADI, EMILIA, WEBB, KEVIN F and CLARK, MATT, 2018. Non destructive evaluation of biological cells
  • ABAYZEED, SIDAHMED A, SMITH, RICHARD J, WEBB, KEVIN F, SOMEKH, MICHAEL G, SEE, CHUNG W, 2017. Optics Express. 25(25), 31552-31567
  • PEREZ-COTA, FERNANDO, SMITH, RICHARD J, MORADI, EMILIA, LEIJA, ANAID LUGO, VELICKOVIC, KSENIJA, MARQUES, LEONEL, WEBB, KEVIN F, SYMONDS, MICHAEL E, SOTTILE, VIRGINIE and ELSHEIKHA, HANNY, 2017. Cell imaging by phonon microscopy: sub-optical wavelength ultrasound for non-invasive imaging: Imaging & Microscopy Imaging & Microscopy. 2017(3),
  • P脡REZ-COTA, F., SMITH, R. J., MORADI, E., WEBB, K. and CLARK, M., 2016. Journal of Physics: Conference Series. 684(1), 012003
  • ABAYZEED, SIDAHMED A., SMITH, RICHARD J., WEBB, KEVIN F., SOMEKH, MICHAEL G. and SEE, CHUNG W., 2016. Sensors and Actuators B: Chemical. 235, 627-635
  • P脡REZ-COTA F, SMITH RJ, MORADI E, MARQUES L, WEBB KF and CLARK M, 2016. Scientific reports. 6, 39326
  • P脡REZ-COTA, FERNANDO, SMITH, RICHARD J., MORADI, EMILIA, MARQUES, LEONEL, WEBB, KEVIN F. and CLARK, MATT, 2016. Scientific Reports. 6, 39326
  • PEREZ-COTA, F., SMITH, R. J., MORADI, E., MARQUES, L., WEBB, K. F. and CLARK, M., 2016. Sci Rep. 6, 39326
  • PEREZ-COTA, FERNANDO, SMITH, RICHARD J, MORADI, EMILIA, WEBB, K and CLARK, MATT, 2016. Thin-film transducers for the detection and imaging of Brillouin oscillations in transmission on cultured cells
  • WEBB, K. F., 2015. Journal of Microscopy. 257(1), 8-22
  • SMITH, R. J., COTA, F. P., MARQUES, L., CHEN, X. S., ARCA, A., WEBB, K., AYLOTT, J., SOMEKH, M. G. and CLARK, M., 2015. Optically excited nanoscale ultrasonic transducers: Journal of the Acoustical Society of America Journal of the Acoustical Society of America. 137(1), 219-227
  • HU, F., SOMEKH, M. G., ALBUTT, D. J., WEBB, K., MORADI, E. and SEE, C. W., 2015. Scientific Reports. 5,
  • P脡REZ-COTA, F., SMITH, R. J., MORADI, E., MARQUES, L., WEBB, K. F. and CLARK, M., 2015. Applied Optics. 54(28), 8388-8398
  • CLARK, M., MARQUES, L., MORADI, E., PEREZ-COTA, F., SMITH, R. J. and WEBB, K. F., 2015.
  • CLARK, MATTHEW, MARQUES, LEONEL, PEREZ-COTA, FERNANDO, SMITH, RICHARD J, WEBB, KEVIN F and OTHERS, 2015. Cell imaging with sub-optical wavelength ultrasound In: Asia Communications and Photonics Conference. ASu5H-1
  • CLARK, MATT, MARQUES, LEONEL, MORADI, EMILIA, PEREZ-COTA, FERNANDO, SMITH, RICHARD J and WEBB, KEVIN F, 2015. Cell imaging using sub-optical wavelength ultrasonics
  • PEREZ-COTA, F., SMITH, R. J., MORADI, E., MARQUES, L., WEBB, K. F. and CLARK, M., 2015. Appl Opt. 54(28), 8388-98
  • PETROVA, ROSICA S, GREY, ANGUS C, WEBB, KEVIN F, SCHEY, KEVIN L and DONALDSON, PAUL J, 2015. Exposure to osmotic stress increases the insertion of AQP5 in the membranes of cortical fiber cells: implications for the water permeability of lens fiber cells: Investigative Ophthalmology & Visual Science Investigative ophthalmology & visual science. 56(7), 3563-3563
  • SMITH, RICHARD J, COTA, FERNANDO PEREZ, MARQUES, LEONEL, CHEN, XUESHENG, ARCA, AHMET, WEBB, KEVIN, AYLOTT, JONATHON, SOMEKH, MICHEAL G and CLARK, MATT, 2015. Optically excited nanoscale ultrasonic transducers: The Journal of the Acoustical Society of America The Journal of the Acoustical Society of America. 137(1), 219-227
  • WEBB, K. F., 2015. J Microsc. 257(1), 8-22
  • AYLOTT, J., CLARK, M., MARQUES, L., PEREZ-COTA, F., SMITH, R. J. and WEBB, K. F., 2013.
  • PARMENTER CDJ, WANG TT and WEBB KF, 2013. Imaging & Microscopy. 27-32
  • WEBB, KEVIN F, DE FILLIPI, G and JOHNSTON, NA, 2013. Condenser-free Zernike phase contrast imaging for scanning probe microscopy Microsc. Anal. 27, 27-32
  • LEE, DANIEL JAMES, VAGHEFI, EHSAN, WEBB, KEVIN F and FOSS, ALEXANDER JE, 2013. Anatomical and ASL imaging of the retina at 7 T: Proceedings of the International Society of Magnetic Resonance Medicine Proceedings of the International Society of Magnetic Resonance Medicine. 0450
  • GUNNING, S.J., CHUNG, K.K.H., DONALDSON, P.J. and WEBB, K.F., 2012. American Journal of Physiology. Cell Physiology. 303(12), C1252-C1259
  • PARMENTER, C., WANG, T.T. and WEBB, K.F., 2012. Focused ion beam ablation rate tomography In: European Microscopy Congress 2012.
  • PETROVA, R.S., GREY, A.C., WEBB, K.F., SCHEY, K.L. and DONALDSON, P.J., 2012. Aquaporin 5 expression in the lens: A role for a third water channel in the lens In: International Society for Eye Research.
  • WEBB, KF, DONALDSON, PJ and CHUNG, KK, 2012. Am J Physiol Cell Physiol 303.
  • GUNNING, S. J., CHUNG, K. K., DONALDSON, P. J. and WEBB, K. F., 2012. Am J Physiol Cell Physiol. 303(12), C1252-9
  • PARMENTER, C., WANG, T.T. and WEBB, K.F., 2011. Focused ion beam ablation rate tomography In: Cryo Microscopy Group Meeting.
  • MENDOZA-NARANJO, A., BOUMA, G., PEREDA, C., RAMI艛EZ, M., WEBB, K. F., TITTARELLI, A., LO峁擡Z, M. N., KALERGIS, A. M., THRASHER, A. J., BECKER, D. L. and SALAZAR-ONFRAY, F., 2011. Journal of Immunology. 187(6), 3121-3132
  • GUNNING, SIMON, WEBB, K and DONALDSON, PAUL, 2011. Ionic conductance and cataract: Two distinct non-selective cation channels in lens fiber cells
  • WEBB, KEVIN F, ZHANG, JING and SOMEKH, MIKE, 2011. Multimodal imaging of fluid transport in living epithelial sheets
  • DONALDSON, P.J., WEBB, K.F. and DANA, R., 2010. Ionic permeability and currents in the lens. In: DARTT, D.A., BESHARSE, J.C. and DANA, R., eds., Encyclopedia of the Eye 2. Elsevier Science. 477-486
  • WEBB, K.F. and RUSSELL, N.A., 2010. Label-free cellular imaging by wide-field Surface Plasmon Resonance microscopy. In: Journal of General Physiology: Optical Methods in Cell Physiology 136. 1. Society of General Physiologists. 117
  • WEBB, K.F. and RUSSELL, N.A., 2010. Characterisation of primary hippocampal neural networks grown on plasmonic substrates. In: FENS Abstracts 5. Federation of European Neuroscience. 011.60
  • RUSSELL, N.A., WEBB, K.F., PITTER, M.C. and SOMEKH, M.G., 2010. Surface plasmon resonance imaging of neural networks. In: FENS Abstracts 5. Federation of European Neuroscience. 192.48
  • WEBB, KF and RUSSELL, NA, 2010. Label-free Cellular Imaging by Wide-Field Surface Plasmon Resonance Microscopy In: 64th Annual Meeting of the Society-of-General-Physiologists. 13A-13A
  • WEBB, KEVIN F and RUSSELL, NOAH A, 2010. Label-free Cellular Imaging by Wide-Field Surface Plasmon Resonance Microscopy
  • DONALDSON, P.J, CHEE, K.N, LIM, J.C and WEBB, K.F., 2009. Experimental Eye Research. 88(2), 144-150
  • WEBB, K.F. and DONALDSON, P.J., 2009. Investigative Ophthalmology & Visual Science. 50(8), 3808-3818
  • DONALDSON, P. J., CHEE, K. S., LIM, J. C. and WEBB, K. F., 2009. Exp Eye Res. 88(2), 144-50
  • HU, R.G, SUZUKI-KERR, H, WEBB, K.F, RHODES, J.D, COLLISON, D.J, DUNCAN, G and DONALDSON, P.J., 2008. Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens. Experimental eye research. 87(2), 137-46
  • WEBB, K.F and DONALDSON, P.J., 2008. American Journal of Physiology. Cell Physiology. 294(5), C1133-C1145
  • HU, R. G., SUZUKI-KERR, H., WEBB, K. F., RHODES, J. D., COLLISON, D. J., DUNCAN, G. and DONALDSON, P. J., 2008. Exp Eye Res. 87(2), 137-46
  • WEBB, K. F. and DONALDSON, P. J., 2008. Am J Physiol Cell Physiol. 294(5), C1133-45
  • WEBB, K.F, CHEE, K.N, KISTLER, J and DONALDSON, P., 2007. Spatially distinct Cl- influx and efflux pathways interact to maintain lens cell volume In: Experimental Biology 2007, Washington DC, USA. April 28th 鈥� May 2nd, 2007. In FASEB J. 2007 21:774.18
  • BECKER, D.L, WEBB, K.F, THRASIVOULOU, C, LIN, C-C, NADERSHAHI, R, TSAKIRI, N and COOK, J.E., 2007. Journal of Physiology. 585(Pt 3), 711-9
  • WEBB, KEVIN FRANCIS, CHEE, KAA SANDRA NARDIA, KISTLER, JOERG and DONALDSON, PAUL JAMES, 2007. Spatially distinct Cl-influx and efflux pathways interact to maintain lens cell volume: The FASEB Journal The FASEB Journal. 21, 774.18
  • WEBB, KEVIN F, 2006. Spatial variations in the membrane properties of differentiating fibre cells isolated from the rat lens
  • WEBB, K.F and DONALDSON, P.J., 2005. Probing the membrane properties of the differentiating lens fibre cell In: MedSciNZ 2005, Queenstown, NZ. December 3rd-6th, 2005.
  • DONALDSON, PJ, CHEE, KN, WEBB, KF and KISTLER, J, 2005. Spatially Distinct Cl鈥揑nflux and Efflux Pathways Interact to Maintain Lens Volume and Transparency: Investigative Ophthalmology & Visual Science Investigative ophthalmology & visual science. 46(13), 1129-1129
  • WEBB, K.F, MERRIMAN-SMITH, B.R, STOBIE, J.K, KISTLER, J and DONALDSON, P.J., 2004. Investigative Ophthalmology and Visual Science. 45(12), 4400-8
  • WEBB, K.F, MERRIMAN-SMITH, B.R and DONALDSON, P.J., 2004. Cl- influx into rat cortical lens fiber cells is mediated by an outwardly rectifying Cl- conductance that is not ClC-2 or ClC-3 In: MedSciNZ 2004, Queenstown, NZ. November 30th 鈥� December 3rd 2004.
  • WEBB, K.F, CHEE, K.N, KISTLER, J and DONALDSON, P.J., 2004. An emerging model for cell volume regulation in the rat lens: A Focus on the Lens In: Rotorua, NZ. 24-27th August 2004. (official satellite meeting of the 2004 ICER congress).
  • WEBB, K. F., MERRIMAN-SMITH, B. R., STOBIE, J. K., KISTLER, J. and DONALDSON, P. J., 2004. Invest Ophthalmol Vis Sci. 45(12), 4400-8
  • WEBB, K.F, CHEE, K.N, KISTLER, J and DONALDSON, P.J., 2003. Volume regulation in the rat lens: roles for chloride channels and transporters: The Biology of Chloride In: Society for General Physiology Symposium: 3-7th September, Woods Hole, MA, USA. Proceedings in Journal of General Physiology, August 2003.
  • WEBB, K.F, MERRIMAN-SMITH, B.R, KISTLER, J and DONALDSON, P.J., 2002. Chloride conductances in lens fiber cells: A role for CLC3? In: 鈥淭ransport 2002鈥� University of Miami, FL, USA.
  • WEBB, K.F, MERRIMAN-SMITH, B.R, KISTLER, J and DONALDSON, P.J., 2002. Chloride conductances in lens fiber cells: A role for CLC3? In: Association for Research in Vision & Ophthalmology (ARVO) May 5-10th 2002, Ft Lauderdale, FL, USA. Proceedings in Investigative Ophthalmology & Visual Science, May 2002.
  • DONALDSON, P.J, WEBB, K.F, YOUNG, M.A, MERRIMAN-SMITH, B.R and KISTLER, J., 2001. Morphological and molecular evidence for a circulating chloride flux that modulates the volume of the rat lens In: 鈥淢olecular Mechanisms in Cell Volume Regulation鈥�, 22-24 August 2001, Queenstown, NZ.
  • WEBB, K.F, ECKERT, R, KISTLER, J and DONALDSON, P.J., 2001. Mapping the membrane conductance properties of differentiating fiber cells isolated from the rat lens In: International Union of Physiological Sciences (IUPS) Congress August 2001, Christchurch, NZ. In Proceedings of the Physiological Society of New Zealand 20: 2001.
  • WEBB, K.F and DONALDSON, P.J., 2000. Membrane properties of differentiating lens fiber cells in solutions containing calcium In: Physiological Society of New Zealand (PSNZ), December 2000, Auckland, NZ. In Proceedings of the Physiological Society of New Zealand 19:71, 2000.

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