糖心原创

Contact

• workRoom C24 Veterinary Academic Building
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• work0115 951 6454
• fax0115 951 6440
• sabine.totemeyer@nottingham.ac.uk

Biography

Sabine T枚temeyer obtained a Diplom in Biology (roughly equivalent to MSc) in Microbiology, Biochemistry and Plant Physiology from the University of Osnabr眉ck in Germany. She obtained a PhD from the University of Aberdeen in 1997. She then spent two years as a postdoctoral research associate at the Christian de Duve Institute for Medical Sciences, Universit茅 Catholique de Louvain, Brussels, Belgium. This was followed by six years of research in the Cell Biology of Infection Group at the Centre for Veterinary Science, Cambridge Veterinary School, University of Cambridge. She joined the newly formed School of Veterinary Medicine and Science in 2006. She is involved in module convening of the Lymphoreticular Cell Biology (LCB) module in Year 1 & 4 and areas of teaching include cell biology, microbiology, host pathogen interactions, innate immunity, antibody based and molecular diagnostics ass well as Personal and Professional Skills (PPS) and communications skills. Her research interest include the Role of Toll-like receptors (TLRs) in host-bacteria interaction sand the development of complex epithelial tissue culture models. Research focuses on understanding host pathogen interactions in footrot in sheep , listeriosis in cattle and antimicrobial resistance. Her educational research focuses on neurodiversity and EDI, transition and 1st year experience.

Expertise Summary

Sabine T枚temeyer is an Associate Professor in Cellular Microbiology, a convener for the year 1 module Cardiorespiratory System, a Welfare senior tutor and a Neurodiversity Champion

Teaching Summary

I co-convening the Cardiorespiratory modules in year 1 (CAR1007, CAR1011) covering microbiology, immunology and heamatology. I am also involved in teaching PPS and communication skills .

Research Summary

My main interest is in the host-bacterial interactions and the development of complex in vitro tissue culture systems

1. Understanding inflammatory processes in ovine footrot

In England more than 95% of sheep flocks have footrot, with an estimated cost to the UK sheep industry of 拢24-84M per annum. Footrot is caused by bacteria that invade the skin of the foot, and cause pain and separation of the horn from the hoof.

Antibiotic injection early in disease leads to recovery within a few days. In the long term however, it would be preferable to instead use vaccination (currently vaccination is part of a 5 point management plan, rather than a single preventative measure), helping to reduce our reliance on and use of antibiotics.

By studying the local immune defences in the sheep foot and how the bacteria causing footrot are recognised by the immune system, in addition to a complementary molecular analysis of the primary pathogen associated with disease, researchers hope to explain better how the clinical signs of footrot are caused and how the immune system contributes to this. The study will help to inform new approaches to disease management and ultimately disease prevention.

Our projects are funded by BBSRC's Animal Health Research Club (ARC), Capes Brazil & Tertiary Education Trust Fund (tetfund) Nigeria

2. Listeriosis in cattle

The intracellular pathogen Listeria monocytogenes is of major importance to global food security and of public health relevance. L. monocytogenes is foodborne pathogen but also impacts on the reproductive performance of beef and dairy cattle. Infection in ruminants manifests as keratoconjunctivitis or bacteraemia, which can lead to abortion or meningitis, hence causing production losses due to calf losses through abortion and extra veterinary bills, whilst delaying conception, compromising herd health and reducing milk yield after abortion leading to an average cost of 拢630 per abortion in a dairy herd.

Our research focuses on host pathogen interactions in conjunctival and reproductive tissues using tissue and organ culture models.

Other research interests:

• Role of Toll-like receptors (TLRs) in host-bacteria interactions

The innate immune system is of crucial importance for achieving homeostasis in the intestinal environment and the host response to pathogens. Toll-like receptors (TLRs) are a family of membrane receptors that recognize a wide range of pathogen associated molecular patterns (PAMPs). We are studying the modulation of TLR expression and cytokine production due to interactions with probiotics and/or pathogens. 1. Interactions of epithelial intestinal cells with commensals and pathogens (incl Clostridium difficile, Salmonella, Vibrio etc) 2. Interaction of bovine caruncular epithelia cells with abortifacient pathogens Leptospira 3. Effect of probiotics on weaning stress in pigs

• Development of complex epithelial tissue culture models

In research, due to increasing ethical awareness and technical developments, there is an increased need for models that mimic organs and tissues. To achieve better likeness to in vivo situation for the study of disease processes and host we are using and developing complex tissue culture systems 1. bacterial interactions in the intestinal tract, we are developing 3D tissue culture using rotary systems and reduced oxygen concentration. 2. Air interface culture systems 3. Organoid models using feeder cells to sustain tissue biopsies/necropsies

• Educational Research

My areas of interest include 1. Assessment and feedback 2. First year experience (graduate students, international students) 3. Student selection/admission

Recent Publications

• MABONI G, BLANCHARD A, FROSTH S, STEWART C, EMES R and T脰TEMEYER S, 2017. Scientific reports. 7, 45220
• MABONI G, DAVENPORT R, SESSFORD K, BAIKER K, JENSEN TK, BLANCHARD AM, WATTEGEDERA S and ENTRICAN G, 2017. Front. Cell. Infect. Microbiol.
• SHIBANY KA, T脰TEMEYER S, PRATT SL and PAINE SW, 2017. Xenobiotica; the fate of foreign compounds in biological systems. 1-8 (In Press.)
• R. TARLINTON, L.ALDER, J.MORETON, G.MABONI, R.EMES and S.TOTEMEYER, 2016. BMC research notes. 353

• View all publications

Past Research

(1) Role of Toll like receptors in Salmonella infection

My work involves the analysis of bacterial growth and the host immune response mediated by toll like receptors (TLRs) during Salmonella infection in vivo (mouse typhoid model) and in vitro (bone marrow derived macrophages (BMDM) & cell lines). I have investigated the mRNA expression of TLR2, TLR4, and MD2 in spleens and livers of C3H/HeN mice (carrying wild-type TLR4) and C3H/HeJ mice (carrying mutated TLR4) in response to Salmonella infection. During Salmonella infections, TLR4 is activated, leading to increased TLR2 and decreased TLR4 expression (T枚temeyer et al., 2003). I also characterized the expression of a wide range of TLRs, TLR associated molecules and inflammatory mediators in C57/BL6 mice (T枚temeyer et al., 2005). In BMDM in response to Salmonella infection, production of nitric oxide (NO) is fully dependent on the activation of TLR4, while the production of TNFS is only partially TLR4 dependent (Royle et al., 2003). TLR4 plays an important role in SipB independent Salmonella induced cell death via the adapter molecules Trif and Tram (Cook, T枚temeyer et al. 2007). Stimulation of bone marrow derived macrophages with IFNg results in the production of NO in response to the bacterial cell wall component peptidoglycan in a TLR independent, Nod dependent way (T枚temeyer et al 2006).

(2) Adaptation of bacteria to intracellular environment

Array technology was used to investigating how Salmonella adapts to the intracellular macrophage environment. Infection of BMDMs from phox^-/- and TLR4^-/- mice followed by isolation of bacterial RNA allowed to determine the changes occurring in Salmonella gene expression using microarrays. I identified known genes required for the adaptation to an environment containing reactive oxygen species as well as genes of unknown function that might also be critical for S. typhimurium survival within macrophages (Wright, T枚temeyer et al, in preparation).

• MABONI G, BLANCHARD A, FROSTH S, STEWART C, EMES R and T脰TEMEYER S, 2017. Scientific reports. 7, 45220
• MABONI G, DAVENPORT R, SESSFORD K, BAIKER K, JENSEN TK, BLANCHARD AM, WATTEGEDERA S and ENTRICAN G, 2017. Front. Cell. Infect. Microbiol.
• SHIBANY KA, T脰TEMEYER S, PRATT SL and PAINE SW, 2017. Xenobiotica; the fate of foreign compounds in biological systems. 1-8 (In Press.)
• R. TARLINTON, L.ALDER, J.MORETON, G.MABONI, R.EMES and S.TOTEMEYER, 2016. BMC research notes. 353
• SHIBANY KA, T脰TEMEYER S, PRATT SL and PAINE SW, 2016. Pharmacology research & perspectives. 4(5), e00268
• RUTLAND, CATRIN S., DOBBS, HEIDI and TOTEMEYER, SABINE, 2016. JOURNAL OF VETERINARY MEDICAL EDUCATION. 43(4), 372-381
• MABONI, G., FROSTH, S., ASPAN, A. and TOTEMEYER, S., 2016. VETERINARY RECORD. 179(9),
• WARREN J, OWEN AR, GLANVILL A, FRANCIS A, MABONI G, NOVA RJ, WAPENAAR W, REES C and T脰TEMEYER S, 2015. Veterinary microbiology. 179(1-2), 76-81
• DAVENPORT, REBECCA, HEAWOOD, CHRISTOPHER, SESSFORD, KATE, BAKER, MELISSA, BAIKER, KERSTIN, BLACKLAWS, BARBARA, KALER, JASMEET, GREEN, LAURA and TOETEMEYER, SABINE, 2014. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY. 161(1-2), 90-98
• KYDD JH, COBB K, DAVIS R, DEAN R, DRINKWATER J, DUNHAM S, FLYNN R, GRAY C, GROGONO-THOMAS R, HAGAN P, JEFFERIES A, JONES M, OGDEN C, PEARSON G, ROBINSON N, RUTLAND C, TARLINTON R, T脰TEMEYER S, WASON J, WHITTINGTON R, WILLIAMS L and MOSSOP L, 2013. The Veterinary record. 173(14), 346
• TOETEMEYER, SABINE, 2013. VETERINARY RECORD. 173(15), 366-U45
• SHERWOOD, V., KING, E., T脰TEMEYER, S., CONNERTON, I. and MELLITS, K.H., 2012. Journal of General Virology: JGV. 93(3), 618-623
• T脰TEMEYER S, DOBBS H and RUTLAND CS, 2012. Journal of veterinary medical education. 39(2), 180-8
• TARLINTON, RE, YON, L, KLISCH, K, TOTEMEYER, S and GOUGH, KC, 2011. Journal Of Veterinary Medical Education. 38(3), 305-310
• WRIGHT, J.A., T脰TEMEYER, S.S., HAUTEFORT, I., APPIA-AYME, C., ALSTON, M., DANINO, V., PATERSON, G.K., MASTROENI, P., MENAGER, N., ROLFE, M., THOMPSON, A., UGRINOVIC, S., SAIT, L., HUMPHREY, T., NORTHERN, H., PETERS, S.E., MASKELL, D.J., HINTON, J.C.D. and BRYANT, C.E, 2009. Microbiology. 155(9), 2919-2929
• TALBOT, S., T脰TEMEYER, S., YAMAMOTO, M., AKIRA, S., HUGHES, K., GRAY, D., BARR, T., MASTROENI, P., MASKELL, D.J. and BRYANT, C.E., 2009. Immunology. 128(4), 472-483
• SMYTH, T., T脰TEMEYER, S., HAUGLAND, S., WILLERS, C., PETERS, S., MASKELL, D. and BRYANT, C., 2008. FEMS Immunology and Medical Microbiology. 54(3), 339-348
• COOK,, T脰TEMEYER,, STEVENSON,, FITZGERALD,, YAMAMOTO,, AKIRA,, MASKELL, and BRYANT,, 2007. Immunology. 122(2), 222-9
• T脰TEMEYER, S., SHEPPARD, M., LLOYD, A., ROPER, D., DOWSON, C., UNDERHILL, D., MURRAY, P., MASKELL, D. and BRYANT, C., 2006. IFN-纬 enhances production of nitric oxide from macrophages via a mechanism that depends on nucleotide oligomerization domain-2 Journal of Immunology. 176(8), 4804-4810
• SMYTH, TOMOKO, HARRIS, HAYLEY J, BROWN, ANDREW, T脰TEMEYER, SABINE, FARNFIELD, BELINDA A, MASKELL, DUNCAN J, MATSUMOTO, MAKOTO, PLEVIN, ROBIN, ALLDRIDGE, LOUISE C and BRYANT, CLARE E, 2006. Immunology. 117(3), 340-9
• T脰TEMEYER, S., KAISER, P., MASKELL, D.J. and BRYANT, C.E., 2005. Infection and immunity. 73(3), 1873-1878
• JONES, M.A., T脰TEMEYER, S., MASKELL, D.J., BRYANT, C.E. and BARROW, P.A., 2003. Infection and Immunity. 71(5), 2626-2633
• TOTEMEYER, S., FOSTER, N., KAISER, P., MASKELL, D. J. and BRYANT, C. E., 2003. Infection and Immunity. VOL 71(PART 11), 6653-6657
• ROYLE, M.C J., T脰TEMEYER, S., ALLDRIDGE, L.C, MASKELL, D.J and BRYANT, C.E., 2003. Stimulation of Toll-like receptor 4 by lipopolysaccharide during cellular invasion by live Salmonella typhimurium is a critical but not exclusive event leading to macrophage responses. Journal of Immunology (Baltimore, Md. : 1950). 170(11), 5445-54
• DENECKER, GEERTRUI, T脰TEMEYER, SABINE, MOTA, LU脥S J, TROISFONTAINES, PAUL, LAMBERMONT, ISABELLE, YOUTA, CARINE, STAINIER, ISABELLE, ACKERMANN, MARTIN and CORNELIS, GUY R, 2002. Infection and immunity. 70(7), 3510-20
• TOTEMEYER, S. and CORNELIS, G. R., 2002. METHODS IN MICROBIOLOGY. ISSU 31, 263-276
• STAINIER, I, BLEVES, S, JOSENHANS, C, KARMANI, L, KERBOURCH, C, LAMBERMONT, I, T脰TEMEYER, S, BOYD, A and CORNELIS, G R, 2000. Molecular microbiology. 37(5), 1005-18
• BOYD, A P, GROSDENT, N, T脰TEMEYER, S, GEUIJEN, C, BLEVES, S, IRIARTE, M, LAMBERMONT, I, OCTAVE, J N and CORNELIS, G R, 2000. European journal of cell biology. 79(10), 659-71
• LEVINA, N, T脰TEMEYER, S, STOKES, N R, LOUIS, P, JONES, M A and BOOTH, I R, 1999. The EMBO journal. 18(7), 1730-7
• FERGUSON, G P, T脰TEMEYER, S, MACLEAN, M J and BOOTH, I R, 1998. Archives of microbiology. 170(4), 209-18
• T脰TEMEYER, S, BOOTH, N A, NICHOLS, W W, DUNBAR, B and BOOTH, I R, 1998. Molecular microbiology. 27(3), 553-62
• TOTEMEYER, S, BARRETTBEE, K and BOOTH, IR, 1996. MICROBIOLOGY-UK. 142, 2857-2862