糖心原创

Contact

• workRoom C22 Veterinary Academic Building
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• work0115 951 6272
• fax0115 951 6440
• kevin.gough@nottingham.ac.uk

Biography

Kevin Gough graduated with a BSc (Hons) in Biochemistry from the University of Wales, Aberystwyth (1992). He then obtained a PhD in Biochemistry from Aberystwyth, graduating in 1996. His post-doctoral employment includes three years as a research associate at The University of Leicester before becoming a Research Scientist with ADAS. Within 8 years at ADAS, his roles progressed through Senior Research Scientist to Principal Research Scientist and Leader of the ADAS Biotechnology Group. Kevin joined the School of Veterinary Medicine and Science as a Lecturer in Molecular Biochemistry in 2007, became an Associate Professor in 2012, and Professor of Biochemistry and Pathology in 2019. Between 2018 and 2023 I was the Director of UNICAS (a 糖心原创 scheme supporting early stage interdisciplinary collaborations), and from 2022 I have been the Director of Research for the School of Veterinary Medicine and Science.

Expertise Summary

Kevin Gough is Professor of Biochemistry and Pathology within the SVMS.

He has acted as an independent scientific expert on appraisal panels for the Defra TSE Review, for the Food Standards Agency TSE Research Programme Review and for the MRC Prion Research Reviews. He has also been an Editorial Board Member for the journal Scientific Reports.

He has extensive experience of research in Molecular Biology and Biochemistry. Techniques include: prion diagnostics, recombinant antibody production, protein purification, monoclonal antibody production, diagnostic PCR and Q-PCR, enzyme kinetics and immunoassay development, next generation phage display, next generation sequencing, biomarker discovery. He also has extensive experience of project and staff management, contract management, bid preparation, results dissemination, business development and commercialisation.

Research Summary

My research is focused on the development and application of novel molecular diagnostics and therapeutics. This includes targeting a range of pathogens, including prions (BSE and scrapie), viruses,… read more

Selected Publications

• SPILIOTOPOULOS, A, BLOKPOEL FERRERAS, L, DENSHAM, RM, CAULTON, SG, MADDISON, BC, MORRIS, JR, DIXON, JE, GOUGH, KC and DRIVEN, I, 2018. J Biol Chem. 294, 424-436
• NAQID, IBRAHIM A., OWEN, JONATHAN P., MADDISON, BEN C., SPILIOTOPOULOS, ANASTASIOS, EMES, RICHARD D., WARRY, ANDREW, TCHORZEWSKA, MONIKA A., MARTELLI, FRANCESCA, GOSLING, REBECCA J., DAVIES, ROBERT H., LA RAGIONE, ROBERTO M. and GOUGH, KEVIN C., 2016. SCIENTIFIC REPORTS. 6, 24232
• BC MADDISON, J SPIROPOLOUS, CM VICKERY, R LOCKEY, JP OWEN, K BISHOP, CA BAKER and KC GOUGH, 2015. Vet Res. 46, 46
• KC GOUGH, CA BAKER, HA SIMMONS, SA HAWKINS and BC MADDISON, 2015. Vet Res. 46, 40

• View all publications

Research Group Members:

Dr Alison Gray: Research area: the application of next generation phage display technology to reduce animal use in antibody production

Dr Hope Leverett: Research area: the application of next generation phage display technology to reduce animal use in antibody production

Dr Kieran Pitchers: Developing and applying phage display antibody libraries. Aims across projects include to 1) isolate antibodies against viruses for diagnostic and therapeutic application and 2) isolate anti-glycosaminoglycan antibodies as tools to study GAG biology and as diagnostic/drug targeting tools in cancer (see ).

Rachel Cruickshank: Research area: Developing cell models for Alzheimer's disease/canine cognitive dysfunction, screening anti-amyloid beta recombinant antibodies for therapuetic efficacy.

Cerys Stock: Research area: developing a novel phage display technology platform to screen for cell-penetrating peptide-fusion proteins that target cancer cells.

Beth Lawrence: Research area: designing and applying phage ligand libraries to isolate anti-glycosaminoglycan ligands as as tools to study GAG biology and to target cancer cells for drug delivery.

Associated group members:

Dr Jon Owen (ADAS): Research areas: Application of next generation phage display applied to the development of novel ligands.

Keith Bishop (ADAS): Research areas: Prion diagnostics and understanding the role of environmental reservoirs in scrapie transmission.

Dr Karina Bingham (UoN): Research areas: Developing and applying phage display antibody libraries to isolate antibodies against viruses for diagnostic and therapeutic application.

Collaborators:

Dr Ben Maddison (ADAS)

Professor Janet Daly (SVMS, 糖心原创)

Dr Cathy Merry (School of Medicine, 糖心原创)

Dr Robert Atterbury (SVMS, 糖心原创)

Dr Cinzia Allegrucci (SVMS, 糖心原创)

Dr Helen Rees (ADAS)

Dr James Dixon (School of Pharmacy, 糖心原创)

Current Research

My research is focused on the development and application of novel molecular diagnostics and therapeutics. This includes targeting a range of pathogens, including prions (BSE and scrapie), viruses, parasites and bacteria. Non-infectious diseases are also targeted, including Alzheimer's disease and cancer. Research is also carried out into the application of molecular diagnostic methods to monitor endangered wildlife species.

鈥婲ext generation phage display: Our design of novel diagnostics is centred around ligand display systems and particularly the display of recombinant antibody fragments and peptides on filamentous bacteriophage. Peptide phage-display libraries have proved useful for mapping epitopes of monoclonal antibodies, and the isolation of ligands that bind specifically to a range of target molecules. This technology also allows the isolation of peptides capable of disrupting specific protein-protein interactions. In the form of antibody phage-display libraries this technology is a powerful in vitro technique that allows the selection of antibodies with defined properties from vast libraries of binders. This allows the isolation of antibodies with exquisite selectivity between closely related molecules, and by removing the need for immunization it allows the production of antibodies against toxic or non-antigenic molecules. To date, we have applied phage-display methods to isolate ligands against a wide range of molecular targets including host cell surfaces, pathogen surface molecules, proteins, peptides and haptens. In recent years, we have coupled the vast diversity of phage display libraries with the screening power of next generation sequencing, a process called next generation phage display (NGPD). This novel technique allows the highly efficient selection of a wide range of specific ligands.

Protein misfolding diseases. Our main research focus is on prion diseases (transmissible spongiform encephalopathies, TSEs). These are fatal neurological disorders that are marked by long incubation periods. TSEs have been described in a number of mammalian species and include scrapie in sheep and goats, bovine spongiform encephalopathy (BSE) in cattle, chronic wasting disease in deer and elk and Creutzfeldt-Jakob disease (CJD) in humans. These diseases are irreversible and invariably fatal; there is compelling evidence that the BSE agent has crossed the species barrier and is the causal agent of vCJD in humans. According to the protein only hypothesis, the causal agent appears to be novel, an infectious misfolded version (PrP^Sc) of a host protein (PrP^C) which encodes transmissible strain characteristics in the absence of any detectable nucleic acid. Our research includes the elucidation of the routes of scrapie transmission and the environmental fate of the TSE agent, the strain typing of ruminant TSEs to study emerging strains, and the pre-mortem diagnosis of TSE diseases. Using NGPD, we are also developing novel ligands that bind specifically to misfolded proteins involved in Alzheimer's disease and prion diseases, in order to apply them as therapeutic and diagnostic tools.

The development of serodiagnostics and therapeutics to infectious diseases. NGPD is being applied in our research to epitope-map immune responses to disease, both antibody-mediated and cell-mediated, to improve diagnostics and develop novel peptide vaccines. Ongoing research is on a range of viral infection in ruminants and humans (West Nile virus, Rift Valley fever virus, louping ill virus, tick-borne encephalitis virus, dengue virus, and Zika virus). Our research is leading to the development of assays to differentiate closely related infections and also vaccinated from infected animals (so-called DIVA tests). Fasciola hepatica infection of cattle and sheep, this parasite causes livestock mortality and reductions in productivity. Our research aims to develop novel multi-component vaccines. Salmonella and E. coli infections of pigs and poultry. These infections place significant economic burdens on livestock industries and zoonotic serovars represent some of the most common foodborne pathogens affecting human health. Our research aims to identify antimicrobial peptides that can specifically target pathogenic bacterial species.

Targeting cancer. We are applying NGPD to map autoantibody responses to a range of cancers with the aim to develop novel biomarkers. We are also applying the technique in conjunction with a cell penetrating peptide system to develop tumour-specific transduction cassette for efficient drug delivery.

• MAR脥N鈥怣ORENO, A, BENESTAD, S.L., BARRIO, T., PIRISINU, L., ESPINOSA, J. C., TRAN, L., HUOR, A., DI BARI, M.A., ERA脩A, H., MADDISON, B.C., D鈥橝GOSTINO, C., FERN脕NDEZ鈥怋ORGES, N., CANOYRA, S., JEREZ鈥怗ARRIDO, N., CASTILLA, J., SPIROPOULOS, J., BISHOP, K., GOUGH, K.C., NONNO, R., V脜GE, J., ANDR脡OLETTI, O. and TORRES, J.M., 2024. Veterinary Research. 55, 62
• ANGANI, M.T., OWEN, J.P., MADDISON, B.C. and GOUGH, K.C., 2024. Journal of Immunological Methods. 534, 113759
• SPILIOTOPOULOS, A., MAURER, S.K., TSOUMPELI, M.T., BONFANTE, J.A., OWEN, J.P., GOUGH, K.C. and DREVENY, I., 2023. Methods Mol Biol. 2591, 189-208
• DALY, JM, SOON, TL and GOUGH, KC, 2023. Therapeutic phage display-derived single-domain antibodies (VHH) for pandemic preparedness Antibodies. Online,
• KOPYCKA, K., MADDISON, B.C. and GOUGH, K.C., 2023. Scientific Reports. 13, 3452
• TSOUMPELI, M.T., VARGHESE, A., OWEN, J.P., MADDISON, B.C., DALY, J.M. and GOUGH, K.C., 2023. Methods in Molecular Biology. 2702, 467-487
• TSOUMPELI MT, GRAY A, PARSONS AL, SPILIOTOPOULOS A, OWEN JP, BISHOP K, MADDISON BC and GOUGH KC, 2022. Mol Biotechnology. 64, 791-803
• CHONG, A., FOSTER, J.D., GOLDMANN, W., GONZALEZ, L., JEFFREY, M., O'CONNOR, M.J., BISHOP, K., MADDISON, B.C., HOUSTON, E.F., GOUGH, K.C. and HUNTER, N., 2021. Scientific Reports. 11, 11931
• GOUGH KC, BAKER CA, HAWKINS S, SIMMONS H, KONOLD T and MADDISON BC, 2019. Veterinary Record. 184, 97
• SOMERVILLE RA, FERNIE K, SMITH A, BISHOP K, MADDISON BC, GOUGH KC and HUNTER N, 2019. Arch. Virol.. 164, 1135-1145
• READER, RH, WORKMAN, RG, MADDISON, BC and GOUGH, KC, 2019. Molecular Biotechnology. (online),
• RADLEY, EH, LONG, J, GOUGH KC and LAYFIELD R, 2019. Biochem . Soc. Trans.. 47(6), 1949-1962
• HELEN C REES and KEVIN C GOUGH, 2018. Great crested newt eDNA laboratory quality systems, proficiency testing and interpretation of results CIEEM inpractice. 99, 24-27
• LYNSEY R HARPER, LORI LAWSON HANDLEY, CHRISTOPH HAHN, NEIL BOONHAM, HELEN C REES, KEVIN C GOUGH, ERIN LEWIS, IAN P ADAMS, PETER BROTHERTON, SUSANNA PHILLIPS and BERND H脛NFLING, 2018. Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of great crested newt (Triturus cristatus) Ecology and Evolution. 8, 6330-6341
• SPILIOTOPOULOS, A, BLOKPOEL FERRERAS, L, DENSHAM, RM, CAULTON, SG, MADDISON, BC, MORRIS, JR, DIXON, JE, GOUGH, KC and DRIVEN, I, 2018. J Biol Chem. 294, 424-436
• O'CONNOR MJ, BISHOP K, WORKMAN RG, MADDISON BC and GOUGH KC, 2017. Prion. 11, 54-64
• STAVROU, A, DALY, J, MADDISON, B, GOUGH, K and TARLINTON, R, 2017. How is Europe positioned for a re-emergence of Schmallenberg virus? The Veterinary Journal. 222, 52-53
• GOUGH KC, BAKER CA and MADDISON BC, 2017. Veterinary Microbiology. 207, 138-142
• WORKMAN RG, MADDISON BC and GOUGH KC, 2017. Prion. 11, 265-276
• AHMAD K, TWELL D, GOUGH KC and MADDISON KC, 2017. Expression of four S. pneumoniae type 2 polysaccharide biosynthetic enzymes utilising the endogenous Kex2 protease activity in tobacco Pak. J. Pharm. Sci.. 30(2), 439-448
• HELEN C. REES, CLAIRE A. BAKER, DAVID S. GARDNER, BEN C. MADDISON and KEVIN C. GOUGH, 2017. BMC Research Notes. 10, 327
• SACHDEV D, GOUGH KC and FLYNN RJ, 2017. Frontiers in Immunology. 8, 1002
• NAQID, IBRAHIM A., OWEN, JONATHAN P., MADDISON, BEN C., SPILIOTOPOULOS, ANASTASIOS, EMES, RICHARD D., WARRY, ANDREW, TCHORZEWSKA, MONIKA A., MARTELLI, FRANCESCA, GOSLING, REBECCA J., DAVIES, ROBERT H., LA RAGIONE, ROBERTO M. and GOUGH, KEVIN C., 2016. SCIENTIFIC REPORTS. 6, 24232
• NAQID IA, OWEN JP, MADDISON BC, SPILIOTOPOULOS A, EMES RD, WARRY A, FLYNN RJ, MARTELLI F, GOSLING RJ, DAVIES RH, LA RAGIONE RM and GOUGH KC, 2016. Scientific reports. 6, 31186
• GOUGH KC, BISHOP K, SOMERVILLE R and HUNTER N, 2016. F1000Research. 5, 2529
• SULAIMAN AM, ZOLNIERCZYK K, JAPA O, OWEN JP, MADDISON BC, EMES RD, HODGKIN J, GOUGH KC and FLYNN RJ, 2016. PLoS Pathog. 12(11), e1005991
• SPILIOTOPOULOS, A, OWEN, JP, MADDISON, BC, DREVENY, I, REES, HC and GOUGH, KC, 2015. J Immunol Meth. 420, 50-55
• DALY, JM, KING, B, TARLINTON, R, GOUGH, KC, MADDISON, BC and BLOWEY, R, 2015. BMC Vet Res. 11, 56
• BC MADDISON, J SPIROPOLOUS, CM VICKERY, R LOCKEY, JP OWEN, K BISHOP, CA BAKER and KC GOUGH, 2015. Vet Res. 46, 46
• KC GOUGH, CA BAKER, HA SIMMONS, SA HAWKINS and BC MADDISON, 2015. Vet Res. 46, 40
• REES, HC, GOUGH, KC, MIDDLEDITCH, DJ, PATMORE, JRM and MADDISON, BC, 2015. J. Appl. Ecol. 52, 827-831
• SIMMONS, M.M., CHAPLIN, M.J., VICKERY, C.M., SIMON, S., DAVIS, L., DENYER, M., LOCKEY, R., STACK, M.J., O'CONNOR, M.J., BISHOP, K., GOUGH, K.C., MADDISON, B.C., THORNE, L. and SPIROPOULOS, J., 2015. J. Clin. Micro.. 53, 8
• GOUGH KC, MADDISON BC, SHIKOTRA A, MOISEEVA EP, YANG W, JARVIS S and BRADDING P, 2015. Respiratory Research. 16, 86
• KONOLD, T., HAWKINS, S.A.C., THURSTON, L.C., MADDISON, B.C., GOUGH, K.C., DUARTE, T. and SIMMONS, H.A., 2015. Frontiers in Veterinary Science. 2, 32
• GOUGH KC, REES HC, IVES SE and MADDISON BC, 2015. Biology. 4(4), 785-813
• SMITH, C.M, FRY, S.C., GOUGH, K.C., PATEL, A.J.F., GLENN, S., HAWES, W.H., GOLDRICH, M., ROBERTS, I.S., WHITELAM, G.C. and ANDREW. P.W., 2014. PLoSONE. 9(2), e88144
• REES, H.C., GOUGH, K.C., MIDDLEDITCH, D.J., PATMORE, J.R.M. and MADDISON, B.C., 2014. Journal of Applied Ecology.
• REES, H.C., BISHOP, K., MIDDLEDITCH, D.J., PATMORE, J.R.J., MADDISON, B.C. and GOUGH, K.C., 2014. Ecology and Evolution.
• GOUGH, KC, BISHOP, K and MADDISON, BC, 2014. Journal of Clinical Microbiology. 52, 3863-3868
• HAWKINS SA, SIMMONS HA, GOUGH KC and MADDISON BC, 2014. Vet Rec.
• NAQID, I.A., OWEN, J.P., MADDISON, B.C., GARDNER, D.S., FOSTER, N., TCHORZEWSKA, M.A., LA RAGIONE, R.M. and GOUGH, KC, 2014. Animal Feed Science and Technology.
• GOUGH, K.C., BAKER, C.A., REES, H.C., TERRY, L.A., SPIROPOULOS, J., THORNE, L. and MADDISON, B.C., 2012. Journal of Virology. 86(1), 566-571
• MADDISON, B.C. AND GOUGH K.C., 2012. Scrapie dissemination and environmental persistence Goat Vet. Soc. J.. 28, 25-32
• MADDISON, B.C., OWEN, J.P., TAEMA, M.M., SHAW, G. and GOUGH, K.C., 2012. Prion. 6(3), 302鈥�308
• THORNE, L, HOLDER, T, RAMSAY, A, EDWARD, J, TAEMA, M, MADDISON, B C, GOUGH, K C and TERRY, L A, 2012. BMC Vet Res. 8, 223
• TERRY L.A, HOWELLS, L, BISHOP K, BAKER C.A, EVEREST S, THORNE L and MADDISON B.C. AND GOUGH K.C., 2011. Vet. Res.. 42, 65
• GOUGH, K.C and JARVIS, S. AND MADDISON B.C., 2011. Env. Sci. Health, Part B.. 46, 581-589
• GOUGH, K.C., 2011. Biochem. Mol. Biol. Educ.. 39(6), 421-5
• TARLINTON, RE, YON, L, KLISCH, K, TOTEMEYER, S and GOUGH, KC, 2011. Journal Of Veterinary Medical Education. 38(3), 305-310
• TAEMA MM, MADDISON BC, THORNE L, BISHOP K, OWEN J, HUNTER N, BAKER CA, TERRY LA and GOUGH KC, 2011. Molecular Biotechnology. 51(3), 233-9
• FEATHER, L, GOUGH, K and FLYNN, R.J. AND ELSHEIKHA, H., 2010. Parasitology Research. 107, 279-283
• MADDISON B.M, OWEN, J.P, BISHOP, K, SHAW, G and REES H.C. AND GOUGH K.C., 2010. Env. Sci. Technol.. 44, 8503-8508.
• MADDISON, B.C., BAKER, C.A., TERRY, L.A., BELLWORTHY, S.J., THORNE L, and REES, L., 2010. Journal of Virology. 84(21), 11560-1562
• MADDISON, B.C., REES, H.C., BAKER, C.A., TAEMA, M., BELLWORTHY, S.J., THORNE, L., TERRY, L.A. GOUGH, K.C. and GOUGH, K.C., 2010. Journal of Infectious Diseases. 201(11), 1672-1676
• GOUGH KC and MADDISON BC, 2010. Prion. 4(4), 275-82
• MADDISON, B.C., BAKER, C.A., REES, H.C., TERRY, L.A., THORNE, L., BELLWORTHY, S.J., WHITELAM G.C. and AND GOUGH, K.C., 2009. Journal of Virology. 83(16), 58578
• REES, H.C, MADDISON, B.C, OWEN, J.P and WHITELAM, G.C. AND GOUGH, K.C., 2009. Molecular Biotechnology. 41, 254-262
• GOUGH, K.C, BAKER, C.A and TAEMA, M. AND MADDISON, B.C., 2009. Prion. 3(4), 1-4
• GOUGH, K.C, PATEL, S and BAKER, C.A. AND MADDISON, B.C., 2009. J. Agri. Food. Chem.. 57, 9393-9399
• OWEN, J.P., REES, H.C., MADDISON, B.C., TERRY, L.A., THORNE, L., JACKMAN, R., WHITELAM, G.C. and GOUGH, K.C., 2007. Journal of Virology. 81(19), 10532-10539
• MADDISON, B.C., WHITELAM, G.C. and GOUGH, K.C., 2007. Biochemical and Biophysical Research Communications. 353(1), 195-199
• OWEN, JONATHAN P, MADDISON, BEN C, WHITELAM, GARRY C and GOUGH, KEVIN C, 2007. Molecular Biotechnology. 35(2), 161-70
• LA RAGIONE, R.M., PATEL, S., MADDISON, B., WOODWARD, M.J., BEST, A., WHITELAM, G.C. and GOUGH, K.C., 2006. Microbes and Infection. 8(2), 426-433
• MADDISON, B.C., PATEL,S., JAMES, R.F., CONLON, H.E., OIDTMANN, B., BAIER, M., WHITELAM, G.C and GOUGH, K.C., 2005. Journal of Immunological Methods. 306, 202-210.
• K脺HNE, S.A., HAWES, W.S., LA RAGIONE, R.M., WOODWARD, M.J., WHITELAM, G.C. and GOUGH, K.C., 2004. Journal of Clinical Microbiology. 42(7), 2966-2976
• GOUGH, K.C. and WHITELAM, G.C., 2003. Production of Antibodies using Transgenic Plants. In: SINGH, R.P. and JAIWAL, P.K., eds., Plant genetic engineering: Applications and limitations 1. Sci Tech Publishing LLC, U.S.A.. 261-277
• GOUGH, K.C., LI, Y. and WHITELAM, G.C., 2002. Phage-Display Libraries. In: GILMARTIN, P. and BOWLER, C., eds., Molecular Aspects of Plant Biology: Practical Approach Series IRL Press Ltd., Eynsham, Oxford. 221-236
• GOUGH K., ALLISON, G., ROGERS, L. and SMITH, A., 2002. European Journal of Phycology. 37, 419-428
• GOUGH, K.C., HAWES, W.S., KILPATRICK, J. and WHITELAM, G.C., 2001. Plant Cell Reports. 20, 296-300
• BISHOP, K, GOUGH, K, MAHONEY, S, SMITH, A and ROGERS, L, 1999. FEBS Letters. 450(1-2), 57-60
• GOUGH, K C, COCKBURN, W and WHITELAM, G C, 1999. Journal of Virological Methods. 79(2), 169-80
• GOUGH, K C, LI, Y, VAUGHAN, T J, WILLIAMS, A J, COCKBURN, W and WHITELAM, G C, 1999. Journal of Immunological Methods. 228(1-2), 97-108
• ALLISON, G, GOUGH, K, ROGERS, L and SMITH, A, 1997. Molecular and General Genetics. 255(4), 392-9