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School of Pharmacy

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Lee Buttery

Associate Professor in Cell Biology/Tissue Engineering, Faculty of Science

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Biography

I gained a BSc (Hons) degree in Applied Zoology at the University of Leeds in 1987 and MSc (Biomolecular Organization; 1990) and PhD (Medicine/Pathology; 1996) degrees at the University of London. Prior to moving to Nottingham in 2003, I was based largely at the former Royal Postgraduate Medical School, now part of Imperial College London working with late Professor Dame Julia Polak. My PhD focussed on investigating the distributions and possible functions of nitric oxide/nitric oxide synthases in the physiology and pathology of a variety of tissues and organs and I was fortunate enough to publish some of the first papers on the involvement of these molecules in human diseases, notably in the cardiovascular system.

My first postdoctoral position was held jointly between Imperial College and the Royal London Hospital School of Medicine and Dentistry (with Professor Francis Hughes) looking at the roles of the nitric oxide and prostaglandin pathways in bone biology, specifically the osteoblast.

I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. From 1999 onwards my research has been focused primarily on osteoblast biology and approaches to tissue engineer and model bone biology, pathology and repair. At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo.

In 2003 I moved to the University Nottingham and the School of Pharmacy. I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. My research continues to focus on stem cells and osteoblasts and in particular 3D cell culture models and microenvironments. I work on various biochemical and biophysical approaches to investigate and control cell-cell, cell-scaffold interactions.

Coordinator (Nottingham) of EPSRC/MRC CDT in Regenerative Medicine http://www.dtcregen-med.com/

Expertise Summary

Histology, immunocytochemistry, general cell biology, 2D and 3D cell culture (including embryonic stem cell, mesenchymal stem cells and primary tissue-derived cells), holographic optical tweezers (for cell biology)

Teaching Summary

I teach on the Master of Pharmacy (MPharm) and Master of Science / Bachelor of Science (MSci/BSc) in Pharmaceutical Sciences courses. I cover mainly cell biology and physiology.

Modules MPharm;

Year 1. Fundamentals of Pharmacy, Bacterial and Fungal Infections

Year 3. Research Project

Year 4 Future Medicines

Modules MSci/BSc Pharmaceutical Science:

Year 1. Cardiovascular and Haematology

Basic cell biology (structure and organization of the cell and biological macromolecules)

Pharmaceutical microbiology -bacterial pathogens, pathogenesis of bacterial infections, infection control and management

Stem cell biology - basics of stem cell sources/origins, self-renewal and differentiation

Tissue engineering and regenerative medicine

I am also UG Admissions Tutor to the School of Pharmacy and coordinate recruitment activities (open days, offer holder days) and selection of candidates to the MPharm course in particular.

Research Summary

My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. I am involved in a… read more

Selected Publications

  • MORRIS, S.A., GREWAL, S., BARRIOS, F., PATANKAR, S.N., STRAUSS, B., BUTTERY, L., ALEXANDER, M., SHAKESHEFF, K.M. and ZERNICKA-GOETZ, M., 2012. Nature Communications. 3, 673
  • PASCUT, F.C., GOH, H.T., WELCH, N., BUTTERY, L.D., DENNING, C. and NOTINGHER, I., 2011. Biophysical Journal. 100(1), 251-259
  • LANNIEL, M., LU, B., CHEN, Y., ALLEN, S., BUTTERY, L., WILLIAMS, P., HUQ, E. and ALEXANDER, M., 2011. THIN SOLID FILMS. VOL 519(NUMBER 6), 2003-2010
  • MAHLSTEDT, M.M., ANDERSON, D., SHARP, J.S., MCGILVRAY, R., MU脩OZ, M.D., BUTTERY, L.D., ALEXANDER, M.R., ROSE, F.R.A.J. and DENNING, C., 2010. Biotechnology and Bioengineering. 105(1), 130-140

Current Research

My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. I am involved in a number of projects to investigate the influence of 3-D cell-cell, cell-scaffold/matrix interactions and angiogenesis / vasoreactivity on the induction and control of stem cell differentiation.

I also work with optical tweezers to precisely position stem cells to simulate/recreate the stem cell niche and probe the role of cell-cell, cell-cytokine and cell-scaffold interactions in controlling stem cell potency and differentiation.

Other projects and interests include tissue engineering of human bronchiolar tissue (with Professor Felicity Rose/Dr Amanda Tatler, 糖心原创).

Notable collaborations/collaborators include;

Professor Morgan Alexander (Nottingham) - cell-materials interactions

Professor Amir Ghaemmaghami (Nottingham) - osteoblast-macrophage interactions

Professor Matthew Dalby (University of Glasgow) - osteoblasts and cell mechanics/signalling

Dr Glen Kirkham (Nottingham Trent) Optical tweezers, cell patterning, localized signal gradients

Past Research

Worked for 11 years at the Royal Postgraduate Medical School/Imperial College in cell pathology and in particular investigation of the contribution of nitric oxide and nitric oxide synthases to disease processes including human atherosclerosis, sepsis and more latterly bone damage/turnover.

Future Research

3D in vitro model of bone fracture callus - focus on osteoblast-macrophage interactions

Building 3D microenvironments to study early embryogenesis

  • MICHAEL TAYLOR, DAVID SCURR, MATTHIAS LUTOLF, LEE BUTTERY, MISCHA ZELZER and MORGAN ALEXANDER, 2016. Biointerphases. 11(02A301),
  • KIRKHAM GR, BRITCHFORD E, UPTON T, WARE J, GIBSON GM, DEVAUD Y, EHRBAR M, PADGETT M, ALLEN S, BUTTERY LD and SHAKESHEFF K, 2015. Scientific reports. 5, 8577
  • TAYLOR, A. J., RATNER, B. D., BUTTERY, L. D. K. and ALEXANDER, M. R., 2015. Acta Biomaterialia. 14, 70-83
  • SIDNEY LE, KIRKHAM GR and BUTTERY LD, 2014. Stem cells and development. 23(6), 605-17
  • SIDNEY LE, HEATHMAN TRJ, BRITCHFORD ER, ABED A, RAHMAN CV and BUTTERY LDK, 2014. Tissue engineering. Part A. (In Press.)
  • TAYLOR AJ, RATNER BD, BUTTERY LD and ALEXANDER MR, 2014. Acta biomaterialia. 14, 70-83
  • EDWARD LESTER, SELINA V.Y. TANG, ANDREI KHLOBYSTOV, VANESSA LOCZENSKI ROSE, LEE BUTTERY and CLIVE J. ROBERTS, 2013. CrystEngComm. 15(17), 3256-3260
  • QUTACHI O, SHAKESHEFF KM and BUTTERY LDK, 2013. Journal of controlled release : official journal of the Controlled Release Society. 168(1), 18-27 (In Press.)
  • MORRIS, S.A., GREWAL, S., BARRIOS, F., PATANKAR, S.N., STRAUSS, B., BUTTERY, L., ALEXANDER, M., SHAKESHEFF, K.M. and ZERNICKA-GOETZ, M., 2012. Nature Communications. 3, 673
  • ROGERS CM, WOOLLEY TS, CRUWYS SC, BUTTERY LDK, ROSE FRAJ and SHAKESHEFF KM, 2012. The journal of pharmacy and pharmacology. 64(6), 821-31
  • SUKMAWATI, A., CHAN, W. C., GARNETT, M. C. and BUTTERY, L., 2012. Biodegradable microparticles for cell aggregation and proliferation JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE. 6, 371-372
  • KIRKHAM GR, LOVRICS A, BYRNE HM, JENSEN OE, KING JR, SHAKESHEFF KM and BUTTERY LDK, 2012. Integrative biology : quantitative biosciences from nano to macro. 4(12), 1470-7
  • LANNIEL, M., LU, B., CHEN, Y., ALLEN, S., BUTTERY, L., WILLIAMS, P., HUQ, E. and ALEXANDER, M., 2011. THIN SOLID FILMS. VOL 519(NUMBER 6), 2003-2010
  • FOZARD, J.A., KIRKHAM, G.R., BUTTERY, L.D.K., KING, J.R., JENSEN, O.E. and BYRNE, H.M., 2011. BMC Bioinformatics. 12, 396
  • PASCUT, F.C., GOH, H.T., WELCH, N., BUTTERY, L.D., DENNING, C. and NOTINGHER, I., 2011. Biophysical Journal. 100(1), 251-259
  • BUTTERY, L., BIELBY, R., HOWARD, HOWARD, D. and SHAKESHEFF, K., 2011. 3D Cell Culture: Methods and Protocols. In: HAYCOCK, J.W., ed.,
  • LANNIEL, M., HUQ, E., ALLEN, S., BUTTERY, L., WILLIAMS, P.M. and ALEXANDER, M.R., 2011. Soft Matter. 7(14), 6501-6514
  • MAHLSTEDT, M.M., ANDERSON, D., SHARP, J.S., MCGILVRAY, R., MU脩OZ, M.D., BUTTERY, L.D., ALEXANDER, M.R., ROSE, F.R.A.J. and DENNING, C., 2010. Biotechnology and Bioengineering. 105(1), 130-140
  • ADAMS, G.G, BUTTERY, L, STOLNIK, S, MORRIS, G.A, HARDING, S.E and WANG, N., 2010. Biotechnology and Genetic Engineering Reviews. 27, 285-304
  • GOTHARD, D., ROBERTS, S.J., SHAKESHEFF, K.M. and BUTTERY, L.D., 2010. Tissue Engineering. Part C, Methods. 16(4), 583-595
  • GOTHARD, D, ROBERTS, SJ, SHAKESHEFF KM and BUTTERY LD, 2009. Cytotechnology. 61(3), 135-144 (In Press.)
  • WANG, N., ADAMS, G., BUTTERY, L., FALCONE, F.H. and STOLNIK, S., 2009. Journal of Biotechnology. 144(4), 304-312
  • ROBERTS, SCOTT J, HOWARD, DANIEL, BUTTERY, LEE D and SHAKESHEFF, KEVIN M, 2008. British medical bulletin. 86, 7-22
  • HOWARD D, BUTTERY LD, SHAKESHEFF KM and ROBERTS SJ, 2008. Journal of anatomy. 213(1), 66-72
  • DE BANK, P.A., HOU,Q., WARNER, R.M., WOOD, I.V., ALI, B.E., MACNEIL, S., KENDALL, D.A., KELLAM, B.K., SHAKESHEFF, K.M. and BUTTERY, L.D.K., 2007. Biotechnology and Bioengineering. 97(6), 1617-1625
  • HIDALGO-BASTIDA, LA, BARRY, JJA, EVERITT, N. M., ROSE, F. R. A. J., BUTTERY, LD, HALL, IP, CLAYCOMB, WC and SHAKESHEFF, K. M., 2007. Acta Biomaterialia. In Press, Corrected Proof, Available online 26 February 2007, (In Press.)
  • HOU, QP, DE BANK, PA, BUTTERY, LDK and SHAKESHEFF, KM, 2006. Surface engineering of embryonic stem cells and alginate scaffolds for tissue regeneration Tissue Engineering. 12(4), 1015-1016
  • WANG, N., BUTTERY, L., STOLNIK, S. and ADAMS, G., 2006. In-vitro differentiation of mouse embryonic stem cells into pancreatic insulin producing cells JOURNAL OF PHARMACY AND PHARMACOLOGY. VOL 58(NUMB 10), 140
  • HOU, Q., RUTTEN, F.J.M., SMITH, E.F., BRIGGS, D., DAVIES, M.C., BUTTERY, L.D.K., FREEMAN, R. and SHAKESHEFF, K.M., 2005. Surface and Interface Analysis. 37(12), 1077-1081
  • HOU, QINGPU, FREEMAN, RICHARD, BUTTERY, LEE D K and SHAKESHEFF, KEVIN M, 2005. Biomacromolecules. 6(2), 734-40
  • CHRISTODOULOU,I.D., BUTTERY,L.D.K., TAI,G., HENCH,L.L. and POLAK,J.M., 2005. Journal of Biomedical Materials Research. Part B: Applied Biomaterials. 77(2), 431-446
  • CHRISTODOULOU, IOANNIS, BUTTERY, LEE D K, SARAVANAPAVAN, PRIYA, TAI, GUANGPING, HENCH, LARRY L and POLAK, JULIA M, 2005. Journal of Biomedical Materials Research. Part B: Applied Biomaterials. 74(1), 529-37
  • BIELBY, R.C., BOCCACCINI, A.R., POLAK, J.M. and BUTTERY, L.D.K., 2004. Tissue Engineering. 10(9-10), 1518-1525
  • BOURNE, S., POLAK, J.M., HUGHES, S.P.F. and BUTTERY, L.D.K., 2004. Tissue Engineering. 10(5-6), 796-806
  • AFZAL, FAIZA, POLAK, JULIA and BUTTERY, LEE, 2004. Journal of Pathology. 202(4), 503-10
  • TAI, GUANGPING, POLAK, JULIA M, BISHOP, ANNE E, CHRISTODOULOU, IOANNIS and BUTTERY, LEE D K, 2004. Tissue Engineering. 10(9-10), 1456-66
  • CALDER, J D F, BUTTERY, L, REVELL, P A, PEARSE, M and POLAK, J M, 2004. Journal of Bone and Joint Surgery (British). 86(8), 1209-13
  • BUTTERY,L.D.K., MANCINI,L., MORADI-BIDHENDI,N., OSHAUGHNESSY,M.C., POLAK,J.M. and MACINTYRE,I., 2002. . In: BILEZIKIAN,J.P., RAISZ,L. and RODAN,G., eds., Principles of Bone Biology 2nd Edition. 2. Academic Press. 995-1017
  • BUTLER-MANUEL, SIMON A, BUTTERY, LEE D K, A'HERN, ROGER P, POLAK, JULIA M and BARTON, DESMOND P J, 2002. Journal of The Society for Gynaecological Investigation. 9(1), 47-56
  • AGUIRRE, J., BUTTERY, L., O'SHAUGHNESSY, M., AFZAL, F., FERNANDEZ DE MARTICORENA, I., HUKKANEN, M., HUANG, P., MACINTYRE, I. and POLAK, J., 2001. American Journal of Pathology. 158(1), 247-257
  • BUTTERY, L.D.K., BOURNE, S., XYNOS, J.D., WOOD, H., HUGHES, F.J., HUGHES, S.P.F., EPISKOPOU, V. and POLAK, J.M., 2001. Tissue Engineering. 7(1), 89-99
  • XYNOS, I D, EDGAR, A J, BUTTERY, L D, HENCH, L L and POLAK, J M, 2001. Journal of Biomedical Materials Research. 55(2), 151-7

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