糖心原创

Contact

• workRoom D2 Medical School
  University Park
  Nottingham
  NG7 2RD
  UK
• Paula.Scaife@nottingham.ac.uk

Biography

In 2005 I completed my PhD in Reproductive Immunology entitled "The role of decidual T lymphocytes in human pregnancy". This project was carried out at Newcastle University under the supervision of Professor Stephen Robson, Dr Judith Bulmer and Dr Roger Searle and involved investigation, at the cellular level, of human intrauterine immune responses to pregnancy.

Following on from I moved to the 糖心原创 where I worked with Professor David Gardner examining the effect of nutritional programming on kidney regulation of blood pressure. I then moved to Professor Fiona Broughton Pipkin's research group performing investigation of the placental renin-angiotensin system and the role of folic acid in placental development and folate transport within the placenta. Additionally I worked on the WTCCC3 GWAS for pre-eclamspia with Dr Linda Morgan.

Expertise Summary

• Mass spectrometry
• Clinical Measurements
• Placental biology
• Reproductive Immunology
• Molecular biology techniques, including all aspects of quantitative real time PCR from primer design and assay optimisation to analysis and presentation of data.
• Cell and tissue culture, including primary cell isolation (decidual leucoytyes and placental extravillous trophoblast, murine neural cell culture), matrigel based extravillous trophoblast invasion assays. Also culture using trophoblast cell lines including JAR, JEG, BeWo and HTR. Also worked with various continuous cell lines including BCEC1, HD11, HT29, CaCO2, K562, P815, THP-1 and HeLa.
• Tumour spheroid generation using canine osteosarcoma cells D17 and MG63.
• Immunohistochemistry, including immunofluorescence and TUNEL, imaging, all aspects of slide preparation including processing and paraffin wax embedding of tissue and use of microtome and cryostat. Rapid staining protocols for use with laser capture microdissection. Use of BondMax and ThermoShandon automated staining systems and Sequenza staining system.
• Flow cytometry cell cycle analysis using propidium iodide labelling. Additionally I have used FACS to isolate decidual and peripheral blood leucocytes.
• Aseptic techniques.
• Chromium release assays, including working with radioactive isotopes.
• Cytokine analysis using multiplex technology.
• ELISA and ELISPOT assays.

Research Summary

I work for the Clinical, Metabolic and Molecular Physiology research group.

Selected Publications

• PAULA J WILLIAMS, 2016. . In: DAVID N COOPER, ed., eLS John Wiley & Sons.
• ZIAD OMRAN, PAULA WILLIAMS and CYRIL RAUCH, 2015. Symmetry. 7, 1780-1787
• WALSH M, FAIS S, SPUGNINI EP, HARGUINDEY S, ABU IZNEID T, SCACCO L, WILLIAMS P, ALLEGRUCCI C, RAUCH C and OMRAN Z, 2015. Journal of experimental & clinical cancer research. 34(1), 93
• KURLAK, L. O., WILLIAMS, P. J., BULMER, J. N., BROUGHTON PIPKIN, F. and MISTRY, H. D., 2015. Placenta. 36(5), 611-3

• View all publications

• PAULA J WILLIAMS, 2016. . In: DAVID N COOPER, ed., eLS John Wiley & Sons.
• WALSH M, FAIS S, SPUGNINI EP, HARGUINDEY S, ABU IZNEID T, SCACCO L, WILLIAMS P, ALLEGRUCCI C, RAUCH C and OMRAN Z, 2015. Journal of experimental & clinical cancer research. 34(1), 93
• KURLAK, L. O., WILLIAMS, P. J., BULMER, J. N., BROUGHTON PIPKIN, F. and MISTRY, H. D., 2015. Placenta. 36(5), 611-3
• ZIAD OMRAN, PAULA WILLIAMS and CYRIL RAUCH, 2015. Symmetry. 7, 1780-1787
• HENTSCHKE, MARTA R, POLI-DE-FIGUEIREDO, CARLOS E, PINHEIRO DA COSTA, BARTIRA E, KURLAK, LESIA O, WILLIAMS, PAULA J and MISTRY, HITEN D, 2013. Journal of lipid research. 54(10), 2658-64
• WILLIAMS PJ and MORGAN L, 2012. Pharmacogenomics and personalized medicine. 5, 37-51
• CARTWRIGHT JE and WILLIAMS PJ, 2012. The Journal of endocrinology. 214(1), 79-85
• MISTRY HD and WILLIAMS PJ, 2011. Oxidative medicine and cellular longevity. 2011, 841749
• WILLIAMS PJ and BROUGHTON PIPKIN F, 2011. Best practice & research. Clinical obstetrics & gynaecology. 25(4), 405-17
• WILLIAMS PJ, BULMER JN, INNES BA and BROUGHTON PIPKIN F, 2011. Biology of reproduction. 84(6), 1148-53
• MISTRY, H.D., KURLAK, L.O., WILLIAMS, P.J., RAMSAY, M.M., SYMONDS, M.E. and BROUGHTON PIPKIN, F., 2010. Placenta. 31, 401-408
• WILLIAMS PJ, MISTRY HD, INNES BA, BULMER JN and BROUGHTON PIPKIN F, 2010. Placenta. 31(5), 448-55
• BULMER JN, WILLIAMS PJ and LASH GE, 2010. The International journal of developmental biology. 54(2-3), 281-94
• WILLIAMS, P.J., MARTEN, N., WILSON, V., LITTEN-BROWN, J.C., CORSON, A.M., CLARKE, L., SYMONDS, M.E. and MOSTYN, A., 2009. Reproduction: the Journal of the Society for Reproduction and Fertility. 138(3), 609-617
• RYAN RM, GREEN J, WILLIAMS PJ, TAZZYMAN S, HUNT S, HARMEY JH, KEHOE SC and LEWIS CE, 2009. Gene therapy. 16(3), 329-39
• WILLIAMS PJ, SEARLE RF, ROBSON SC, INNES BA and BULMER JN, 2009. Journal of reproductive immunology. 82(1), 24-31
• WILLIAMS PJ, BULMER JN, SEARLE RF, INNES BA and ROBSON SC, 2009. Reproduction (Cambridge, England). 138(1), 177-84
• WILLIAMS, P.J., KURLAK, L.O., PERKINS, A.C., BUDGE, H., STEPHENSON, T., KEISLER, D., SYMONDS, M.E. and GARDNER, D.S., 2007. Kidney International. 72(3), 279-289
• GNANALINGHAM, M.G., WILLIAMS, P., WILSON, V., BISPHAM, J., HYATT, M.A., PELLICANO, A., BUDGE, H., STEPHENSON, T. and SYMONDS, M.E., 2007. Reproduction. 134, 615-623 (In Press.)
• LASH GE, SCAIFE PJ, INNES BA, OTUN HA, ROBSON SC, SEARLE RF and BULMER JN, 2006. Journal of Immunological Methods. 309, 205-208
• SCAIFE PJ, BULMER JN, ROBSON SC, INNES BA and SEARLE RF, 2006. Biology of Reproduction. 75, 562-567