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Ellis O'Neill

Assistant Professor, Faculty of Science

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Biography

Ellis studied Natural Sciences in Cambridge, focusing on Chemistry, Biochemistry and Plant Sciences. He then went on to the John Innes Centre for his PhD, working in Biological Chemistry on carbohydrate enzymes and algae under the supervision of . Ellis then moved to the Scripps Institution of Oceanography at the University of California - San Diego to work on bacterial natural product discovery in the lab of . Ellis joined Oxford, working with on Eukaryotic synthetic biology, before he was awarded a Glasstone Independent Research Fellowship to work on algal natural products, also holding a Junior Research Fellowship at , Oxford. In 2019 Ellis was awarded a Nottingham Research Fellowship to continue his work on algal natural products in the School of Chemistry and was promoted to Assistant Professor in 2022

Teaching Summary

Ellis teaches organic chemistry and biochemistry throughout the undergraduate courses. He lectures on Molecules of Life (CHEM1019), takes organic chemistry tutorials and leads third year lab… read more

Research Summary

Algal Natural Products

Algae are well known to make complex natural products, notable when they are released during a harmful algal bloom. They also encode the capacity for the synthesis of many as yet undiscovered compounds in their genomes. In order to investigate the molecules they maker, we are using high throughput chemoinformatic strategies to discover these new compounds. This involves the growth of a wide range of algae, simple extraction of the cultures and LCMS2. The data is analysed using the pipeline to compare the metabolites produced among the different samples and to compare to the libraries of known compounds. Using all of this information we can prioritise new compounds produced by these algae for purification and structure elucidation. We are currently applying this strategy to investigating the natural products made by euglenoid algae.

Euglena glycomics

Euglena expresses a wide range of , suggesting an unexpectedly high capacity for the synthesis of complex carbohydrates for a single-celled organism. Aside from the enzymes involved in the synthesis of the 尾-glucan storage polysaccharide (paramylon) and for the synthesis of N-glycans, there are many enzymes related to those involved in plant cell wall biosynthesis. Analysis of the sugar nucleotide pool showed that there are the substrates necessary for synthesis of complex polysaccharides. Lectin-and antibody-based profiling of whole cells and extracted carbohydrates shows a complex galactan, xylan and aminosugar based surface. Work is ongoing to characterise the nature of the complex polysaccharides produced by these cells.

Genomics of Euglena

The genome of Euglena is highly complex for a simple free living cell. The total amount of DNA is similar to that of Human cells and there are more genes encoded. It is not clear why Euglena needs all these genes. The aim of this project is to sequence related Euglena, at transcriptomic, genomic and metagenomic levels. Euglena controls gene expression at post transcriptionally, meaning that the transcriptome at any given time is a good representation of the genome, and avoids the difficulties of sequencing their large complex genome. Comparing the gene between different species will help us to understand the capacity of these cells.

Metabolite profiling

Euglena cells have a 鈥媍omplex metabolism, able to grow using photosynthesis, osmotrophy or eukaryovory. Euglena contain a secondary plastid, derived from a green algae, and acquired by a free living ancestor. E. gracilis can survive after destruction of this plastid, unlike other photosynthetic organisms. In order to understand this complex metabolism in Euglena we are using a range of metabolic profiling techniques. this has revealed the different pathways used by Euglena under alternative conditions and may be used to optimise growth for production of a range of products.

Recent Publications

  • O'NEILL, ELLIS C., 2023. Glycosylated proteins in the protozoan alga Euglena gracilis: a proteomic approach FEMS MICROBIOLOGY LETTERS. 370,
  • GANESAN, A, ALDHOLMI, MOHAMMED, AHMAD, RIZWAN, CARRETERO-MOLINA, DANIEL, PEREZ-VICTORIA, IGNACIO, MARTIN, JESUS, REYES, FERNANDO, GENILLOUD, OLGA, GOURBEYRE, LEA, GEFFLAUT, THIERRY, CARLSSON, HANNE, MAKLAKOV, ALEXEI, FIELD, ROBERT A, O'NEILL, ELLIS, WILKINSON, BARRIE and O'CONNELL, MARIA, 2022. Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis. Angewandte Chemie (International ed. in English).
  • EBENEZER, THANKGOD ECHEZONA, LOW, ROSS S., O'NEILL, ELLIS CHARLES, HUANG, ISHUO, DESIMONE, ANTONIO, FARROW, SCOTT C., FIELD, ROBERT A., GINGER, MICHAEL L., GUERRERO, SERGIO ADRIAN, HAMMOND, MICHAEL, HAMPL, VLADIMIR, HORST, GEOFF, ISHIKAWA, TAKAHIRO, KARNKOWSKA, ANNA, LINTON, ERIC W., MYLER, PETER, NAKAZAWA, MASAMI, CARDOL, PIERRE, SANCHEZ-THOMAS, ROSINA, SAVILLE, BARRY J., SHAH, MAHFUZUR R., SIMPSON, ALASTAIR G. B., SUR, AAKASH, SUZUKI, KENGO, TYLER, KEVIN M., ZIMBA, PAUL V., HALL, NEIL and FIELD, MARK C., 2022. Euglena International Network (EIN): Driving euglenoid biotechnology for the benefit of a challenged world BIOLOGY OPEN. 11(11),
  • SCHORN, MICHELLE A., VERHOEVEN, STEFAN, RIDDER, LARS, HUBER, FLORIAN, ACHARYA, DEEPA D., AKSENOV, ALEXANDER A., ALETI, GAJENDER, MOGHADDAM, JAMSHID AMIRI, ARON, ALLEGRA T., AZIZ, SAEFUDDIN, BAUERMEISTER, ANELIZE, BAUMAN, KATHERINE D., BAUNACH, MARTIN, BEEMELMANNS, CHRISTINE, BEMAN, J. MICHAEL, BERLANGA-CLAVERO, MARIA VICTORIA, BLACUTT, ALEX A., BODE, HELGE B., BOULLIE, ANNE, BREJNROD, ASKER, BUGNI, TIM S., CALTEAU, ALEXANDRA, CAO, LIU, CARRION, VICTOR J., CASTELO-BRANCO, RAQUEL, CHANANA, SHAURYA, CHASE, ALEXANDER B., CHEVRETTE, MARC G., COSTA-LOTUFO, LETICIA V., CRAWFORD, JASON M., CURRIE, CAMERON R., CUYPERS, BART, DANG, TAM, DE ROND, TRISTAN, DEMKO, ALYSSA M., DITTMANN, ELKE, DU, CHAO, DROZD, CHRISTOPHER, DUJARDIN, JEAN-CLAUDE, DUTTON, RACHEL J., EDLUND, ANNA, FEWER, DAVID P., GARG, NEHA, GAUGLITZ, JULIA M., GENTRY, EMILY C., GERWICK, LENA, GLUKHOV, EVGENIA, GROSS, HARALD, GUGGER, MURIEL, GUILLEN MATUS, DULCE G., HELFRICH, ERIC J. N., HEMPEL, BENJAMIN-FLORIAN, HUR, JAE-SEOUN, IORIO, MARIANNA, JENSEN, PAUL R., KANG, KYO BIN, KAYSSER, LEONARD, KELLEHER, NEIL L., KIM, CHUNG SUB, KIM, KI HYUN, KOESTER, IRINA, KOENIG, GABRIELE M., LEAO, TIAGO, LEE, SEOUNG RAK, LEE, YI-YUAN, LI, XUANJI, LITTLE, JESSICA C., MALONEY, KATHERINE N., MAENNLE, DANIEL, MARTIN H., CHRISTIAN, MCAVOY, ANDREW C., METCALF, WILLAM W., MOHIMANI, HOSEIN, MOLINA-SANTIAGO, CARLOS, MOORE, BRADLEY S., MULLOWNEY, MICHAEL W., MUSKAT, MITCHELL, NOTHIAS, LOUIS-FELIX, O'NEILL, ELLIS C., PARKINSON, ELIZABETH I., PETRAS, DANIEL, PIEL, JORN, PIERCE, EMILY C., PIRES, KARINE, REHER, RAPHAEL, ROMERO, DIEGO, ROPER, M. CAROLINE, RUST, MICHAEL, SAAD, HAMADA, SAENZ, CARMEN, SANCHEZ, LAURA M., SORENSEN, SOREN JOHANNES, SOSIO, MARGHERITA, SUSSMUTH, RODERICH D., SWEENEY, DOUGLAS, TAHLAN, KAPIL, THOMSON, REGAN J., TOBIAS, NICHOLAS J., TRINDADE-SILVA, AMARO E., VAN WEZEL, GILLES P., WANG, MINGXUN, WELDON, KELLY C., ZHANG, FAN, ZIEMERT, NADINE, DUNCAN, KATHERINE R., CRUESEMANN, MAX, ROGERS, SIMON, DORRESTEIN, PIETER C., MEDEMA, MARNIX H. and VAN DER HOOFT, JUSTIN J. J., 2021. NATURE CHEMICAL BIOLOGY. 17(4), 363-368

Ellis teaches organic chemistry and biochemistry throughout the undergraduate courses. He lectures on Molecules of Life (CHEM1019), takes organic chemistry tutorials and leads third year lab projects. He also supervises final year projects within his group.

  • O'NEILL, ELLIS C., 2023. Glycosylated proteins in the protozoan alga Euglena gracilis: a proteomic approach FEMS MICROBIOLOGY LETTERS. 370,
  • GANESAN, A, ALDHOLMI, MOHAMMED, AHMAD, RIZWAN, CARRETERO-MOLINA, DANIEL, PEREZ-VICTORIA, IGNACIO, MARTIN, JESUS, REYES, FERNANDO, GENILLOUD, OLGA, GOURBEYRE, LEA, GEFFLAUT, THIERRY, CARLSSON, HANNE, MAKLAKOV, ALEXEI, FIELD, ROBERT A, O'NEILL, ELLIS, WILKINSON, BARRIE and O'CONNELL, MARIA, 2022. Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis. Angewandte Chemie (International ed. in English).
  • EBENEZER, THANKGOD ECHEZONA, LOW, ROSS S., O'NEILL, ELLIS CHARLES, HUANG, ISHUO, DESIMONE, ANTONIO, FARROW, SCOTT C., FIELD, ROBERT A., GINGER, MICHAEL L., GUERRERO, SERGIO ADRIAN, HAMMOND, MICHAEL, HAMPL, VLADIMIR, HORST, GEOFF, ISHIKAWA, TAKAHIRO, KARNKOWSKA, ANNA, LINTON, ERIC W., MYLER, PETER, NAKAZAWA, MASAMI, CARDOL, PIERRE, SANCHEZ-THOMAS, ROSINA, SAVILLE, BARRY J., SHAH, MAHFUZUR R., SIMPSON, ALASTAIR G. B., SUR, AAKASH, SUZUKI, KENGO, TYLER, KEVIN M., ZIMBA, PAUL V., HALL, NEIL and FIELD, MARK C., 2022. Euglena International Network (EIN): Driving euglenoid biotechnology for the benefit of a challenged world BIOLOGY OPEN. 11(11),
  • SCHORN, MICHELLE A., VERHOEVEN, STEFAN, RIDDER, LARS, HUBER, FLORIAN, ACHARYA, DEEPA D., AKSENOV, ALEXANDER A., ALETI, GAJENDER, MOGHADDAM, JAMSHID AMIRI, ARON, ALLEGRA T., AZIZ, SAEFUDDIN, BAUERMEISTER, ANELIZE, BAUMAN, KATHERINE D., BAUNACH, MARTIN, BEEMELMANNS, CHRISTINE, BEMAN, J. MICHAEL, BERLANGA-CLAVERO, MARIA VICTORIA, BLACUTT, ALEX A., BODE, HELGE B., BOULLIE, ANNE, BREJNROD, ASKER, BUGNI, TIM S., CALTEAU, ALEXANDRA, CAO, LIU, CARRION, VICTOR J., CASTELO-BRANCO, RAQUEL, CHANANA, SHAURYA, CHASE, ALEXANDER B., CHEVRETTE, MARC G., COSTA-LOTUFO, LETICIA V., CRAWFORD, JASON M., CURRIE, CAMERON R., CUYPERS, BART, DANG, TAM, DE ROND, TRISTAN, DEMKO, ALYSSA M., DITTMANN, ELKE, DU, CHAO, DROZD, CHRISTOPHER, DUJARDIN, JEAN-CLAUDE, DUTTON, RACHEL J., EDLUND, ANNA, FEWER, DAVID P., GARG, NEHA, GAUGLITZ, JULIA M., GENTRY, EMILY C., GERWICK, LENA, GLUKHOV, EVGENIA, GROSS, HARALD, GUGGER, MURIEL, GUILLEN MATUS, DULCE G., HELFRICH, ERIC J. N., HEMPEL, BENJAMIN-FLORIAN, HUR, JAE-SEOUN, IORIO, MARIANNA, JENSEN, PAUL R., KANG, KYO BIN, KAYSSER, LEONARD, KELLEHER, NEIL L., KIM, CHUNG SUB, KIM, KI HYUN, KOESTER, IRINA, KOENIG, GABRIELE M., LEAO, TIAGO, LEE, SEOUNG RAK, LEE, YI-YUAN, LI, XUANJI, LITTLE, JESSICA C., MALONEY, KATHERINE N., MAENNLE, DANIEL, MARTIN H., CHRISTIAN, MCAVOY, ANDREW C., METCALF, WILLAM W., MOHIMANI, HOSEIN, MOLINA-SANTIAGO, CARLOS, MOORE, BRADLEY S., MULLOWNEY, MICHAEL W., MUSKAT, MITCHELL, NOTHIAS, LOUIS-FELIX, O'NEILL, ELLIS C., PARKINSON, ELIZABETH I., PETRAS, DANIEL, PIEL, JORN, PIERCE, EMILY C., PIRES, KARINE, REHER, RAPHAEL, ROMERO, DIEGO, ROPER, M. CAROLINE, RUST, MICHAEL, SAAD, HAMADA, SAENZ, CARMEN, SANCHEZ, LAURA M., SORENSEN, SOREN JOHANNES, SOSIO, MARGHERITA, SUSSMUTH, RODERICH D., SWEENEY, DOUGLAS, TAHLAN, KAPIL, THOMSON, REGAN J., TOBIAS, NICHOLAS J., TRINDADE-SILVA, AMARO E., VAN WEZEL, GILLES P., WANG, MINGXUN, WELDON, KELLY C., ZHANG, FAN, ZIEMERT, NADINE, DUNCAN, KATHERINE R., CRUESEMANN, MAX, ROGERS, SIMON, DORRESTEIN, PIETER C., MEDEMA, MARNIX H. and VAN DER HOOFT, JUSTIN J. J., 2021. NATURE CHEMICAL BIOLOGY. 17(4), 363-368
  • O'NEILL, ELLIS, 2020. Mining Natural Product Biosynthesis in Eukaryotic Algae MARINE DRUGS. 18(2),
  • EBENEZER, THANKGOD E., ZOLTNER, MARTIN, BURRELL, ALANA, NENAROKOVA, ANNA, NOV脕K VANCLOV脕, ANNA M. G., PRASAD, BINOD, SOUKAL, PETR, SANTANA-MOLINA, CARLOS, O鈥橬EILL, ELLIS, NANKISSOOR, NERISSA N., VADAKEDATH, NITHYA, DAIKER, VIKTOR, OBADO, SAMSON, SILVA-PEREIRA, SARA, JACKSON, ANDREW P., DEVOS, DAMIEN P., LUKE艩, JULIUS, LEBERT, MICHAEL, VAUGHAN, SUE, HAMPL, VLADIM螉R, CARRINGTON, MARK, GINGER, MICHAEL L., DACKS, JOEL B., KELLY, STEVEN and FIELD, MARK C., 2019. BMC Biology. 17(1), 11
  • O鈥橬EILL, ELLIS C., SCHORN, MICHELLE, LARSON, CHARLES B. and MILL脕N-AGUI脩AGA, NATALIE, 2019. Critical Reviews in Microbiology. 45(3), 255-277
  • INWONGWAN, SAHUTCHAI, KRUGER, NICHOLAS J., RATCLIFFE, R. GEORGE and O鈥橬EILL, ELLIS C., 2019. Metabolites. 9(6), 115
  • WAGSTAFF, BEN A., HEMS, EDWARD S., REJZEK, MARTIN, PRATSCHER, JENNIFER, BROOKS, ELLIOT, KUHAUDOMLARP, SAKONWAN, O'NEILL, ELLIS C., DONALDSON, MATTHEW I., LANE, STEVEN, CURRIE, JOHN, HINDES, ANDREW M., MALIN, GILL, MURRELL, J. COLIN and FIELD, ROBERT A., 2018. Biochemical Society Transactions.
  • CR脺SEMANN, MAX, O鈥橬EILL, ELLIS C., LARSON, CHARLES B., MELNIK, ALEXEY V., FLOROS, DIMITRIOS J., DA SILVA, RICARDO R., JENSEN, PAUL R., DORRESTEIN, PIETER C. and MOORE, BRADLEY S., 2017. Journal of Natural Products. 80(3), 588-597
  • KELLY, STEVEN, IVENS, ALASDAIR, MOTT, G. ADAM, O'NEILL, ELLIS, EMMS, DAVID, MACLEOD, OLIVIA, VOORHEIS, PAUL, TYLER, KEVIN, CLARK, MATTHEW, MATTHEWS, JACQUELINE, MATTHEWS, KEITH and CARRINGTON, MARK, 2017. Genome Biology and Evolution. 9(8), 2093-2109
  • INWONGWAN, S., O'NEILL, E. C., KRUGER, N. J. and RATCLIFFE, R. G., 2017. 13C Labelling for metabolic flux analysis in heterotrophic: Phycologia Phycologia. 56(4), 84-84
  • O鈥橬EILL, ELLIS, KUHAUDOMLARP, SAKONWAN, REJZEK, MARTIN, FANGEL, JONATAN, ALAGESAN, KATHIRVEL, KOLARICH, DANIEL, WILLATS, WILLIAM and FIELD, ROBERT, 2017. Biology. 6(4), 45
  • O鈥橬EILL, E. C., 2017. . In: Reference Module in Chemistry, Molecular Sciences and Chemical Engineering Elsevier.
  • O鈥橬EILL, ELLIS C. and KELLY, STEVEN, 2017. Critical Reviews in Biotechnology. 37(6), 779-802
  • O'NEILL, ELLIS C., PERGOLIZZI, GIULIA, STEVENSON, CLARE E. M., LAWSON, DAVID M., NEPOGODIEV, SERGEY A. and FIELD, ROBERT A., 2017. Carbohydrate Research. 451, 118-132
  • O鈥橬EILL, E. C., SAALBACH, G. and FIELD, R. A., 2016. Methods in Enzymology. 576, 99-120
  • O'NEILL, ELLIS C., SAALBACH, GERHARD and FIELD, ROBERT A., 2016. Methods in Enzymology. 576, 99-120
  • O鈥橬EILL, ELLIS C. and FIELD, ROBERT A., 2015. Carbohydrate Research. 403(0), 23-37
  • O鈥橬EILL, ELLIS C, TRICK, MARTIN, HENRISSAT, BERNARD and FIELD, ROBERT A, 2015. Euglena: Perspectives in Science Perspectives in Science. 6, 84-93
  • O'NEILL, E. C., STEVENSON, C. E. M., TANTANARAT, K, LATOUSAKIS, D., DONALDSON, M. I., REJZEK, M, NEPOGODIEV, S. A., LIMPASENI, T, FIELD, R. A. and LAWSON, D. M., 2015. Journal of Biological Chemistry. 290, 29834-29853
  • O'NEILL, ELLIS C., STEVENSON, CLARE E. M., PATERSON, MICHAEL J., REJZEK, MARTIN, CHAUVIN, ANNE-LAURE, LAWSON, DAVID M. and FIELD, ROBERT A., 2015. Proteins: Structure, Function, and Bioinformatics. 83(9), 1742-1749
  • TANG, XIAOYU, LI, JIE, MILL脕N-AGUI脩AGA, NATALIE, ZHANG, JIA JIA, O'NEILL, ELLIS C., UGALDE, JUAN A., JENSEN, PAUL R., MANTOVANI, SIMONE M. and MOORE, BRADLEY S., 2015. ACS Chemical Biology. 10(12), 2841-2849
  • O'NEILL, ELLIS C., TRICK, MARTIN, HILL, LIONEL, REJZEK, MARTIN, DUSI, RENATA G., HAMILTON, CHRIS J., ZIMBA, PAUL V., HENRISSAT, BERNARD and FIELD, ROBERT A., 2015. The transcriptome of: Molecular Biosystems Molecular Biosystems. 11(10), 2808-2820
  • WAGSTAFF, B. A., REJZEK, M., PESNOT, T., TEDALDI, L. M., CAPUTI, L., O'NEILL, E. C., BENINI, S., WAGNER, G. K. and FIELD, R. A., 2015. Enzymatic synthesis of nucleobase-modified UDP-sugars: scope and limitations: Carbohydrate Research Carbohydrate Research. 404, 17-25
  • TANTANARAT, KRIT, O'NEILL, ELLIS C., REJZEK, MARTIN, FIELD, ROBERT A. and LIMPASENI, TIPAPORN, 2014. Process Biochemistry. 49(1), 84-89
  • O'NEILL, ELLIS C., RASHID, ABDUL M., STEVENSON, CLARE E. M., HETRU, ANNE-CLAIRE, GUNNING, A. PATRICK, REJZEK, MARTIN, NEPOGODIEV, SERGEY A., BORNEMANN, STEPHEN, LAWSON, DAVID M. and FIELD, ROBERT A., 2014. Chemical Science. 5(1), 341-350
  • O'NEILL EC and FIELD RA, 2013. Nature Chemistry. 5, 642-643
  • TANTANARAT, K., REJZEK, M., O'NEILL, E. C., RUZANSKI, C., HILL, L., FAIRHURST, S. A., LIMPASENI, T. and FIELD, R. A., 2012. Carbohydrate Research. 358, 12-18

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