糖心原创

Contact

• workRoom C03 Bioenergy and Brewery
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• Ivan.Campeotto@nottingham.ac.uk

Biography

Career summary:

• Senior Lecturer in Biochemistry at Nottingham Trent University
• Wellcome Trust ISSF - Early Career Research Fellow at the Leicester Institute of Structural and Chemical Biology.
• BTP Research Fellow at Pembroke College, University of Oxford.
• Research Associate at the University of Oxford.
• Research Associate at Imperial College London.
• Research Associate at King's College London.
• PhD in protein crystallography at the Astbury Centre for Structural Molecular Biology, University of Leeds with Prof. Simon Philips and Prof. Arwen Pearson
• Degree and MRes in Industrial Biotechnology at the University of Padua (Italy)

Other roles:

Member of the CCP4 WG1 and WG2, Honorary Fellow at LISCB (University of Leicester), Visiting Lecturer (NTU), Editor Board Member of Scientific Reports and Frontiers in Microbiology

Expertise Summary

My group currently focuses on structure-based drug design and structure-guided vaccine development in the fields of Neglected Tropical Diseases (NTDs) and of emerging viruses, with main focus on Chagas disease.

To achieve this, we use protein crystallography as the main research tool in combination with cryo-EM, 2D NMR, HDX-MS and complementary biophysical techniques (ITC, BLI, SPR, DSF, DSC, CD etc.). My group has access to the Diamond Light Source (DLS) MX beamlines, Research Complex at Harwell and other synchrotrons in France (Soleil) and Germany (Petra and DESY). I am also involved in methods development for in situ serial crystallography, to trap intermediates of enzymes from human pathogens in collaboration with the VMXi team at DLS.

We use several expression systems for recombinant protein production: Escherichia coli, Vibrio natrigens, HEK293F cells, sf9 cells, Leishmania tarantulae and more recently Tobacco plants.

Teaching Summary

I am module leader for the UG course on "Fundamental in Immunology" (BIOS2010) which will start in October 2023 and will have particular focus on host-pathogen interactions and vaccines.

I am also module leader for the PG module "Research skills in Microbial Biotechnology" (BIOS4125).

Research Summary

My main research stream lies in the design and production of immunogens from human and animal pathogens, for the generation of monoclonal antibodies, which we test for diagnostic and therapeutic… read more

Recent Publications

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Current Research

My main research stream lies in the design and production of immunogens from human and animal pathogens, for the generation of monoclonal antibodies, which we test for diagnostic and therapeutic applications. After this initial step, we proceed with the determination of antigen-antibody complexes, which are used to guide the design and development of vaccine prototypes via epitopes conjugation to engineered Virus-Like-Particles (VLPs), upon "a la carte" computational epitope crafting.

I was successful in this approach and designed, produced and characterized a heat stable variant of the leading protein candidate for a blood-stage malaria vaccine, called RH5, which lead to a patent with myself as co-inventor (PCT/GB2017/052608), when I was at the University of Oxford, which has been licensed to the Serum Institute of India in 2022, for human trials in children in Gambia and Burkina Faso (trial NCT05357560).

Current Lab members: Oliver Boakye (PhD student), Sam Skinner (PhD student), Alicia Richardson (MRes), Christopher Waugh (Senior Research Technician).

Alumni :

• Dr Sagar Batra (PDRA at University of Bristol)
• Master student Iqra Kaleem (Oxford Expression Technologies)
• Master student Sadia Purba (Freelance Medical writer)
• Master student Ahmad Jazuli
• Master student Nelson Igbokwe (NHS)
• Master student Hirak Naitik Tailor (PhD student at Center of Molecular Medicine Cologne, Germany)
• Master student Raizel Fernandes (NHS)
• Master student Agata Kowalska (R&D at Reckitt)
• Master student Maria Morra (Wellcome Sanger Institute, Cambridge)
• BSc student Camilla Huemert (PhD student, Imperial College London)
• BS student Deborah Goma (Ilumina)

• SAGAR BATRA, FRANCISCO OLMO, TIMOTHY J. RAGAN, MERVE KAPLAN,, VALERIA CALVARESI, ASGER MELDGAARD, CLAUDIA LANCEY, MAHYA ASSADIPAPARI, CUIFENG YING, WESTON B. STRUWE, EMMA L. HESKETH, JOHN M. KELLY, LEA BARFOD and IVAN CAMPEOTTO, 2025. Nature Communications. 16,
• SAGAR BATRA,, CHRIS WAUGH, GEMMA HARRIS, FREDERICK D MUSKETT, TIBERIU M GIANGA, ROHANAH HUSSAIN, GIULIANO SILIGARDI,, PASCAL MERTENS, LAURENCE TURPINS, MALLORY DRAYE, BARRIE ROONEY, MICHAEL A MILES, TAPAN BHATTACHARYYA and IVAN CAMPEOTTO, 2025. bioRXiv.
• THOMAS E HARRISON, NAWSAD ALAM, BRENDAN FARRELL, DORIS QUINKERT, AMELIA M LIAS, LLOYD D W KING, LEA K BARFOD, SIMON J DRAPER, IVAN CAMPEOTTO and MATTHEW K HIGGINS, 2024. EMBO Molecular Medicine.
• KIERAN G PITCHERS, OLIVER D BOAKYE and IVAN CAMPEOTTO, 2024. Pathogens.
• KING, LLOYD D. W., PULIDO, DAVID, BARRETT, JORDAN R., DAVIES, HANNAH, QUINKERT, DORIS, LIAS, AMELIA M., SILK, SARAH E., PATTINSON, DAVID J., DIOUF, ABABACAR, WILLIAMS, BARNABAS G., MCHUGH, KIRSTY, RODRIGUES, ANA, RIGBY, CASSANDRA A., STRAZZA, VERONICA, SUURBAAR, JONATHAN, REES-SPEAR, CHLOE, DABBS, REBECCA A., ISHIZUKA, ANDREW S., ZHOU, YU, GUPTA, GAURAV, JIN, JING, LI, YUANYUAN, CARNROT, CECILIA, MINASSIAN, ANGELA M., CAMPEOTTO, IVAN, FLEISHMAN, SAREL J., NOE, AMY R., MACGILL, RANDALL S., KING, C. RICHTER, BIRKETT, ASHLEY J., SOISSON, LORRAINE A., LONG, CAROLE A., MIURA, KAZUTOYO, ASHFIELD, REBECCA, SKINNER, KATHERINE, HOWARTH, MARK, BISWAS, SUMI and DRAPER, SIMON J., 2024. Cell Reports Medicine.
• MIKOLAJEK, H., SANCHEZ-WEATHERBY, J., SANDY, J., GILDEA, R. J., CAMPEOTTO, I., CHERUVARA, H., CLARKE, J. D., FOSTER, T., FUJII, S., PAULSEN, I. T., SHAH, B. S. and HOUGH, M. A., 2023. IUCrJ. 10(Pt 4), 420-429
• CAMPEOTTO, I., GALAWAY, F., MEHMOOD, S., BARFOD, L. K., QUINKERT, D., KOTRAIAH, V., PHARES, T. W., WRIGHT, K. E., SNIJDERS, A. P., DRAPER, S. J., HIGGINS, M. K. and WRIGHT, G. J., 2020. mBio. 11(5),
• WARSZAWSKI, S., DEKEL, E., CAMPEOTTO, I., MARSHALL, J. M., WRIGHT, K. E., LYTH, O., KNOP, O., REGEV-RUDZKI, N., HIGGINS, M. K., DRAPER, S. J., BAUM, J. and FLEISHMAN, S. J., 2020. Proteins. 88(1), 187-195
• CAMPEOTTO, I., LEBEDEV, A., SCHREURS, A. M. M., KROON-BATENBURG, L. M. J., LOWE, E., PHILLIPS, S. E. V., MURSHUDOV, G. N. and PEARSON, A. R., 2018. Sci Rep. 8(1), 14876
• CAMPEOTTO, I., GOLDENZWEIG, A., DAVEY, J., BARFOD, L., MARSHALL, J. M., SILK, S. E., WRIGHT, K. E., DRAPER, S. J., HIGGINS, M. K. and FLEISHMAN, S. J., 2017. Proc Natl Acad Sci U S A. 114(5), 998-1002
• SCHUSTER, C. F., BELLOWS, L. E., TOSI, T., CAMPEOTTO, I., CORRIGAN, R. M., FREEMONT, P. and GRUNDLING, A., 2016. Sci Signal. 9(441), ra81
• CAMPEOTTO, I., ZHANG, Y., MLADENOV, M. G., FREEMONT, P. S. and GRUNDLING, A., 2015. J Biol Chem. 290(5), 2888-901
• CAMPEOTTO, I., PERCY, M. G., MACDONALD, J. T., FORSTER, A., FREEMONT, P. S. and GRUNDLING, A., 2014. J Biol Chem. 289(41), 28054-69
• DANIELS, A. D., CAMPEOTTO, I., VAN DER KAMP, M. W., BOLT, A. H., TRINH, C. H., PHILLIPS, S. E., PEARSON, A. R., NELSON, A., MULHOLLAND, A. J. and BERRY, A., 2014. ACS Chem Biol. 9(4), 1025-32
• THWAITES, T., NOGUEIRA, A. T., CAMPEOTTO, I., SILVA, A. P., GRIESHABER, S. S. and CARABEO, R. A., 2014. J Biol Chem. 289(44), 30426-30442
• CORRIGAN, R. M., CAMPEOTTO, I., JEGANATHAN, T., ROELOFS, K. G., LEE, V. T. and GRUNDLING, A., 2013. Proc Natl Acad Sci U S A. 110(22), 9084-9
• CAMPEOTTO, I. and ACEVEDO-ROCHA, C. G., 2013. EMBO Rep. 14(4), 299-301
• CAMPEOTTO, I., BOLT, A. H., HARMAN, T. A., DENNIS, C., TRINH, C. H., PHILLIPS, S. E., NELSON, A., PEARSON, A. R. and BERRY, A., 2010. J Mol Biol. 404(1), 56-69
• CAMPEOTTO, I., CARR, S. B., TRINH, C. H., NELSON, A. S., BERRY, A., PHILLIPS, S. E. and PEARSON, A. R., 2009. Acta Crystallogr Sect F Struct Biol Cryst Commun. 65(Pt 11), 1088-90