ࡱ> WYX#` ^1bjbjmm .> dTTd /LdL///////$0hS3h,/-,/Y/   / /  .,hj-X pA@2-^,-\o/0/,3|3 j-3j-@ ,/,/ /d d D lDd d l$ NCMH PhDTutorial 2: Further Viscosity Persistence length Lp and mass per unit length ML Is a measure or chain flexibility - see the D2DBT7 lecture notes "Physical Biochemistry. The mass per unit length ML depends on the saccharide repeat unit and the extent of branching. The Bushin-Bohdanecky method1 This is one of the most popular methods for estimating chain persistence lengths particularly for semi-flexible polymers, and has been applied to range of polysaccharides from charged polysaccharides including xanthan2, sodium hyaluronate3 as well as galactomannans4 and certain neutral polysaccharides such as cereal (-glucans5. In its simplest form, the Bohdanecky method (or Bushin-Bohdanecky method) involves plotting  EMBED Equation.3 versus  EMBED Equation.3  and from the slope Lp/ML can be calculated using the following relation and tabulated values29 of the coefficient B0:  EMBED Equation.3  B0 is ~ 1.025 (ref. 30), the Flory-Fox constant  EMBED Equation.3  is ~ 2.86 x 1023 mol-1 . References Bohdanecky, M. Macromolecules 1983, 16, 1483-1493 and Bushin, S.; Tsvetkov, V.; Lysenko, E.; Emelianov, V. Vysokomol Soedin 1981, 23A, 2494-2503 Sato, T.; Norisuye, T.; Fujita, H. Macromolecules 1984, 17, 2696-2700. Mendichi, R.; Soltes, L.; Schierone, A. G. Biomacromolecules 2003, 4, 1805-1810. Patel, T. R.; Picout, D. R.; Ross-Murphy, S. B.; Harding, S. E. Biomacromolecules 2006, 7, 3513-3520. Gomez, C.; Navarro, A.; Manzanares, P.; Horta, A.; Carbonell, J. V. Carbohyd. Polym. 1997, 32, 7-15. Question 1. Describe in simple terms what you understand by (a) the mass per unit length ML of a polysaccharide and (b) the persistence length Lp. Question 2. Give a table of about 10 literature values for the mass per unit length ML and persistence lengths Question 3. The data in Table 1 describe the change in intrinsic viscosity with molecular weight for g-irradiated xyloglucan samples. Table 1. 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