ࡱ> JHK` !bjbjss .4       4||||4T4(8888$ h"h9   88W 8 8V@  8 d(|:  ,m0,x[#[#[# (444 444 444       NCMH PhDTutorial 1: Viscosity Consult:  HYPERLINK "http://www.nottingham.ac.uk/ncmh/lecture_notes/D2DBT1/D2DBT1_Viscositylecture.ppt" http://www.nottingham.ac.uk/ncmh/lecture_notes/D2DBT1/D2DBT1_Viscositylecture.ppt or go to  HYPERLINK "http://www.nottingham.ac.uk/ncmh" http://www.nottingham.ac.uk/ncmh then go to the lecture notes download page, then click on D2DBT1 Viscositylecture.ppt Question 1: The following data describe data from an Ostwald capillary viscometer for a newly purified protein called roverein. The solvent contained 33.3% dioxane and 0.03M KCl at pH 7.0, T = 20.00oC. C is the protein concentration; (/(o is the ratio of the solution density to that of solvent. C (g/ml) (/(o Flow time readings (sec) 0.00000 1.0000 398.1, 398.2, 398.2 0.00417 1.0011 439.1, 439.1, 439.1 0.00685 1.0019 467.6, 467.6, 467.5 0.00844 1.0023 485.8, 485.8, 485.7 evaluate [(] and KH from a Huggins plot evaluate [h] and KK from a Kraemer plot evaluate [h] from a Solomon-Ciuta plot In each case plot using ORIGIN and give the error estimates for each parameter. What would you conclude about roverein under these conditions? Question 2. The data in Table 1 describe the change in intrinsic viscosity with molecular weight for g-irradiated xyloglucan samples. Table 1. Hydrodynamic data for native and g-irradiated xyloglucans in phosphate-chloride buffer, pH6.8, I=0.1 Xyloglucan sample[(] (mL/g)10-4 x Mw (g/mol)!*+7   9 : ʨʨ|iX!hQhRuB*CJOJQJph$hRuhRu5B*CJOJQJphh55B*CJOJQJphhRumH sH &jh`o0hRu5UmH sH h`o0hRu0J5mH sH &jh`o0hRu5UmH sH jhRu5UmH sH h55mH sH h5mH sH hRu5mH sH hRuhRu5mH sH  ! e f  D h  & Fgd5^gdRugdRu!     _ ` e f ϻs_sJss(hRuhRuB*CJH*OJQJaJph' jhhQhRuB*CJOJQJphhRuB*CJOJQJph$hQhRuB*CJH*OJQJph' jrhQhRuB*CJOJQJph$hQhRuB*CJH*OJQJph'hQhRu5B*CJOJQJ\ph!hRu5B*CJOJQJ\ph!hQhRuB*CJOJQJphh5B*CJOJQJph < > 0 2 $dh<<$Ifa$gd5$0dh^`0a$gdRu$a$gdRugdRugd5 & FgdRuh^hgd5    < @ R T  . 2 n p ~ ~ଜveYQFhV,hRumH sH hRumH sH hRuhRu6mH sH !h5h5B*CJOJQJph$hRuhRu5B*CJOJQJph$hQhRu5B*CJOJQJphh55B*CJOJQJph!hQhRuB*CJOJQJphh5B*CJOJQJph(hRuhRuB*CJH*OJQJaJphhRuB*CJOJQJph!hRuhRuB*CJOJQJphHɺ{eS?S+&hV,hRu56B*H*mH phsH &hV,hRu56B*H*mH phsH #hV,hRu56B*mH phsH +hV,hRu56B*CJaJmH phsH . jhhV,hRu5B*CJaJmH phsH (hV,hRu5B*CJaJmH phsH "hV,hRu56CJaJmH sH hRu56CJaJmH sH hV,hRu5mH sH hRu5mH sH hRumH sH hV,hRumH sH hV,hRuOJQJmH sH         + 0 2 6 7 F K M Q R a f h l m {  rhRuB*CJOJQJph-hV,hRuB*CJaJmH nHphsH tHhV,hRumH sH hV,hRuB*mH phsH hV,hRuCJaJmH sH 3hV,hRu56B*CJaJmH nHphsH tHU hV,hRu5B*mH phsH #hV,hRu56B*mH phsH )XG-0405 3570.0 5.0XG-10210 1027.0 1.0XG-20170 1015.8 0.3XG-30140 1012.7 1.0XG-40135 59.7 1.0XG-50100 56.0 0.4XG-7075 54.5 0.3 Evaluate the MHKS a coefficient. 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